STORAGE-STABLE LIQUID DETERGENT OR CLEANING AGENT CONTAINING PROTEASE AND LIPASE

• US 20130071910A1
• Filed: 11/12/2012
• Published: 03/21/2013
• Est. Priority Date: 05/12/2010
• Status: Abandoned Application

• Current Assignee: BASF SE
• Original Assignee: Henkel AG & Co KGaA
• Inventors: Wieland, Susanne, Maurer, Karl-Heinz, O'Connell, Timothy
• Application Number:
  US13/674,170
• Publication Number:
  US 20130071910A1
• Time in Patent Office: Days
• US Class Current: 435/263
• CPC Class Codes: C11D 3/38618 Protease or amylase in liquid compositions only
• CPC Class Codes: C11D 3/38627 containing lipase
• CPC Class Codes: C11D 3/38681 Chemically modified or immobilised enzymes
• CPC Class Codes: C12N 9/54 bacteria being Bacillus

Abstract

The object is to improve the storage stability of a liquid washing or cleaning agent comprising a protease and lipase with regard to lipolytic activity. This is achieved through the use of a protease comprising an amino acid sequence that is at least 80% identical to the amino acid sequence set out in SEQ ID NO. 1 and that has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the sequence corresponding to SEQ ID NO. 1.

Claims

1. A liquid washing or cleaning agent comprising:

(a) a protease selected from the group of proteases consisting of;

i) a protease comprising an amino acid sequence that is at least 80% identical to the amino acid sequence set out in SEQ ID NO. 1 and that has the amino acid glutamic acid (E) or aspartic acid (D) at position 99 in the sequence corresponding to SEQ ID NO. 1;

ii) a protease comprising an amino acid sequence that is at least 80% identical to the amino acid sequence set out in SEQ ID NO. 1 and that has the amino acid asparagine (N) or glutamine (Q) at position 99 in the sequence corresponding to SEQ ID NO. 1;

iii) a protease comprising an amino acid sequence that is at least 80% identical to the amino acid sequence set out in SEQ ID NO. 1 and that has the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the sequence corresponding to SEQ ID NO. 1;

(b) a lipase.

2. The washing or cleaning agent according to claim 1, wherein the protease additionally has at least one of the following amino acids in the sequence corresponding to SEQ ID NO. 1:

(a) threonine at position 3 (3T),

(b) isoleucine at position 4 (4I),

(c) alanine, threonine or arginine at position 61 (61A, 61T or 61R),

(d) aspartic acid or glutamic acid at position 154 (154D or 154E),

(e) proline at position 188 (188P),

(f) methionine at position 193 (193M),

(g) isoleucine at position 199 (199I),

(h) aspartic acid, glutamic acid or glycine at position 211 (211D, 211E or 211G),

(i) combinations of amino acids (a) to (h).

4. The washing or cleaning agent according to claim 1, wherein the lipase is included in an amount from 1×

10<sup>−

8</sup> to 5 wt. %, relative to active protein, and/or the protease is included in an amount from 1×

10<sup>−

8</sup> to 5 wt. %, relative to active protein.

5. The washing or cleaning agent according to claim 1, wherein it additionally encompasses a component selected from

i. anionic and/or polyanionic substance,

ii. cationic and/or polycationic substance,

iii. substance containing hydroxyl and/or polyhydroxyl group(s).

6. The washing or cleaning agent according to claim 1, wherein it encompasses at least one further ingredient selected from the group consisting of phosphonate, surfactant, builder, non-aqueous solvent, acid, water-soluble salt, thickening agent and combinations thereof.

7. The washing or cleaning agent according to claim 1, wherein it encompasses at least one further enzyme, in particular a protease, amylase, cellulase, hemicellulase, mannanase, tannase, xylanase, xanthanase, xyloglucanase, β

-glucosidase, pectinase, carrageenase, perhydrolase, oxidase, oxidoreductase or a lipase, and mixtures thereof.

8. A method for cleaning textiles or hard surfaces, wherein a washing or cleaning agent according to claim 1 is applied in at least one process step.

9. The method according to claim 8, wherein the lipase is present in the washing liquor in a concentration from 0.00075 to 0.03 wt. %, and/or the protease is present in the washing liquor in a concentration from 0.00075 to 0.03 wt. %.

10. The method according to claim 8, wherein it is performed at a temperature between 10°

C. and 50°

C.

3. A liquid washing or cleaning agent comprising

(a) a protease selected from the group consisting of

a. protease comprising an amino acid sequence corresponding to SEQ ID NO. 2 or SEQ ID NO. 3 or SEQ ID NO. 4 or SEQ ID NO. 5 or SEQ ID NO. 6 or SEQ ID NO. 7 or SEQ ID NO. 8;

b. protease comprising an amino acid sequence that is modified in at least one position in comparison to SEQ ID NO. 2 or SEQ ID NO. 3 or SEQ ID NO. 4 or SEQ ID NO. 5 or SEQ ID NO. 6 or SEQ ID NO. 7 or SEQ ID NO. 8, the modification in the sequence corresponding to SEQ ID NO. 1 being selected from the group consisting of;

i. threonine at position 3 (3T),

ii. isoleucine at position 4 (4I),

iii. alanine, threonine or arginine at position 61 (61A, 61T or 61R),

iv. aspartic acid or glutamic acid at position 154 (154D or 154E),

v. proline at position 188 (188P),

vi. methionine at position 193 (193M),

vii. isoleucine at position 199 (199I),

viii. aspartic acid, glutamic acid or glycine at position 211 (211D, 211E or 211G),

ix. combinations of amino acids (i) to (viii);

(b) a lipase.