LACTATE SENSOR

• US 20130075277A1
• Filed: 09/24/2012
• Published: 03/28/2013
• Est. Priority Date: 09/26/2011
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Yamaguchi, Takehiro, Kaneda, Hisashi
• Granted Patent: US 8,999,126 B2
• Time in Patent Office: Days
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: G01N 2333/904 acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

A lactate sensor capable of accurately measuring a lactate concentration in a short period of time. The lactate sensor includes an insulating substrate, an electrode system including at least a working electrode and a counter electrode provided on the substrate, and a reagent layer provided on the electrode system. The reagent layer contains lactate oxidase, it mediator, and N-(2-acetamide)-2-aminoethanesulfonic acid.

Claims

1. A lactate sensor comprising an insulating substrate, an electrode system including at least a working electrode and a counter electrode provided on the substrate, and a reagent layer provided on the electrode system,

wherein the reagent layer contains lactate oxidase, a mediator, and N-(2-acetamide)-2-aminoethanesulfonic acid.

2. The lactate sensor according to claim 1, wherein the reagent layer further contains a sugar selected from the group consisting of oligosaccharides and polysaccharides.

3. The lactate sensor according to claim 2, wherein the sugar is dextran.

4. The lactate sensor according to claim 1, wherein the reagent layer further contains a layered inorganic compound and a surfactant.

5. The lactate sensor according to claim 1, wherein the mediator is selected from the group consisting of potassium ferricyanide, cytochrome C, pyrroloquinoline quinone, NAD+, NADP+, copper complexes, ruthenium compounds, and phenazine methosulfate and derivatives thereof.

6. The lactate sensor according to claim 5, wherein the mediator is a ruthenium compound, or a ruthenium compound and phenazine methosulfate or it derivative thereof.

7. The lactate sensor according to claim 5, wherein the phenazine methosulfate derivative is 1-methoxy-5-methyl phenazinium methyl sulfate.

12. A lactate concentration measurement system for measuring a lactate concentration in a sample, the system comprising:

the lactate sensor of claim 1,

a means for applying voltage to the electrode system of the lactate sensor, and

a means for measuring a current in the electrode system.

8. A method for measuring a lactate concentration, comprising:

bringing a sample into contact with a reagent containing lactate oxidase and N-(2-acetamide)-2-aminoethanesulfonic acid, and

electrochemically measuring a reaction between lactate in the sample and the lactate oxidase via a mediator.

9. The measurement method according to claim 8, carried out using the lactate sensor,

wherein the lactate sensor comprises an insulating substrate, an electrode system including at least a working electrode and a counter electrode provided on the substrate, and a reagent layer provided on the electrode system,

wherein the reagent layer contains lactate oxidase, a mediator, and N-(2-acetamide)-2-aminoethanesulfonic acid.

10. The measurement method according to claim 9, comprising:

applying voltage to the electrode system after the contact,

measuring;

a value of a response current discharged when the voltage is applied, and

calculating a lactate concentration in the sample based on the value of a response current.

11. A method for producing a lactate sensor, the method comprising forming a reagent layer containing lactate oxidase, a mediator, and N-(2-acetamide)-2-aminoethanesulfonic acid on an electrode system provided on an insulating substrate, the electrode system including at least a working electrode and a counter electrode.