Apparatus and Method for Detecting and Quantifying Analytes in Solution

• US 20130075614A1
• Filed: 11/05/2012
• Published: 03/28/2013
• Est. Priority Date: 08/02/2011
• Status: Active Grant

• Current Assignee: Roc8sci Co.
• Original Assignee: Roc8sci Co.
• Inventors: Hartley, Frank Thomas
• Granted Patent: US 8,541,743 B2
• Time in Patent Office: Days
• US Class Current: 250/339.07
• CPC Class Codes: A61B 2562/0238 Optical sensor arrangements for performing transmission measurements on body tissue
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/6816 Ear lobe
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0259 Monolithic
• CPC Class Codes: G01J 3/10 Arrangements of light sources specially adapted for spectrometry or colorimetry
• CPC Class Codes: G01J 3/26 using multiple reflection, e.g. Fabry-Perot interferometer, variable interference filters
• CPC Class Codes: G01J 3/2803 using photoelectric array detector
• CPC Class Codes: G01J 3/42 Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry beam switching arrangements G01J3/08
• CPC Class Codes: G01J 5/34 using capacitors, e.g. pyroelectric capacitors
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/3144 for oxymetry
• CPC Class Codes: G01N 2021/317 Special constructive features
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/35 using infrared light G01N21/39 takes precedence
• CPC Class Codes: G01N 2201/08 Optical fibres; light guides

Abstract

A method for identifying and quantifying one or more analytes included in a sample comprising a background solvent is disclosed. The present invention locates a sample fluid at a sample region by virtue of a sample holder that comprises work-hardened silver halide. The sample fluid at the sample region is then spectrally characterized via a mid-infrared spectrometer.

Claims

1. An apparatus comprising:

(1) a spectrometer comprising;

(i) a source operable for providing mid-infrared radiation to a sample region;

(ii) a wavelength discriminator operable for receiving a composite light signal from the sample region and spatially dispersing the composite light signal as a plurality of wavelength signals; and

(iii) a plurality of detectors, each of the plurality of detectors operable for generating one of a plurality of first electrical signals based on one of the plurality of wavelength signals; and

(2) a sample holder, the sample holder being operable for locating a sample fluid at the sample region, the sample holder being dimensioned and arranged to enable the sample fluid to receive mid-infrared radiation and provide the composite light signal.

2. The apparatus of claim 1 wherein the sample holder includes a first region that is operable for drawing the sample fluid into the sample holder via capillary action.

3. The apparatus of claim 1 wherein the sample holder includes a sample cell that comprises work-hardened silver halide.

4. The apparatus of claim 3 wherein the sample cell includes a portion of a conduit that is operable for conveying the sample fluid through the sample region, the portion of the conduit comprising work-hardened silver halide.

5. The apparatus of claim 3 wherein the sample cell comprises a chamber operable for containing the sample fluid, the chamber comprising work-hardened silver halide.

6. The apparatus of claim 1 wherein at least one of the plurality of detectors comprises:

(i) a plate, wherein the plate comprises an absorber operable for converting incident mid-infrared radiation to heat;

(ii) an electrode disposed on a substrate, the electrode and the plate collectively defining a parallel-plate capacitor whose capacitance is based on a first separation between the electrode and the plate; and

(iii) an actuator operable for changing the first separation in response to a change in the temperature of the actuator, the actuator comprising a first bi-material element having a first end and a second end, the first end being mechanically coupled with the plate and the second end being substantially immovable with respect to the substrate, wherein a change in the temperature of the first bi-material element induces motion of the first end with respect to the substrate;

wherein the plate and the actuator are thermally coupled to enable transfer of heat from the plate to the actuator, and wherein the actuator and the substrate are thermally coupled to enable transfer of heat from the actuator to the substrate.

7. The apparatus of claim 1 wherein the source is dimensioned and arranged to provide the mid-infrared radiation as a pulse of mid-infrared radiation.

8. The apparatus of claim 7 wherein the source emits the mid-infrared radiation in response to a drive signal that is an electrical pulse.

9. The apparatus of claim 1 further comprising:

(3) a processor; and

(4) a database comprising a plurality of spectra for each of a first chemical constituent and a second chemical constituent, wherein the sample comprises the first chemical constituent and the second chemical constituent;

wherein the processor is operable for receiving the plurality of wavelength signals and providing an output signal that is based on the plurality of wavelength signals and the plurality of spectra.

10. The apparatus of claim 1 further comprising:

(3) a first optical fiber operable for conveying mid-infrared radiation, the first optical fiber comprising silver halide, the first optical fiber being operatively coupled with each of the source and the first region; and

(4) a second optical fiber, the second optical fiber comprising silver halide, the second optical fiber operable for conveying the composite light signal from the first region to the wavelength discriminator.

11. An apparatus comprising:

(1) a spectrometer comprising;

(i) a source operable for providing mid-infrared radiation;

(ii) a wavelength discriminator operable for spatially dispersing a composite light signal into a plurality of wavelength signals; and

(iii) a plurality of detectors, each of the plurality of detectors operable for generating one of a plurality of first electrical signals based on one of the plurality of wavelength signals; and

(2) a sample holder that includes a sample cell that comprises work-hardened silver halide, the sample holder operable for locating a sample fluid such that the sample fluid (a) receives mid-infrared radiation from the source and (b) provides the composite light signal to the wavelength discriminator such that the composite light signal includes the mid-infrared radiation and spectral information based on the sample fluid.

12. The apparatus of claim 11 wherein the sample cell includes a portion of a conduit that is operable for conveying the sample fluid through the sample region, the portion of the conduit comprising work-hardened silver halide.

13. The apparatus of claim 11 wherein the sample cell comprises a chamber operable for containing the sample fluid, the chamber comprising work-hardened silver halide.

14. The apparatus of claim 13 wherein the sample cell includes a first region that is operable for drawing the sample fluid into the sample holder via capillary action, the first region comprising work-hardened silver halide.

15. The apparatus of claim 11 further comprising:

(3) a first optical fiber operable for conveying mid-infrared radiation, the first optical fiber comprising a first material that is substantially transparent for mid-infrared radiation, the first optical fiber being operatively coupled with each of the source and the first region; and

(4) a second optical fiber, the second optical fiber comprising the first material, the second optical fiber operable for conveying the composite light signal from the first region to the wavelength discriminator.

16. An apparatus comprising:

(1) a spectrometer comprising;

(i) a source operable for providing mid-infrared radiation;

(ii) a first optical fiber operable for conveying mid-infrared radiation from the source to a sample region, the first optical fiber comprising silver halide;

(iii) a second optical fiber operable for conveying a composite light signal comprising mid-infrared radiation from the sample region to a wavelength discriminator, the second optical fiber comprising silver halide;

(iv) the wavelength discriminator, the wavelength discriminator being operable for receiving the composite light signal and spatially dispersing it as a plurality of wavelength signals; and

(v) a plurality of detectors, each of the plurality of detectors operable for generating one of a plurality of first electrical signals based on one of the plurality of wavelength signals; and

(2) a sample holder, the sample holder being operable for locating a sample fluid at the sample region, the sample holder being dimensioned and arranged to enable the sample fluid to receive mid-infrared radiation and provide the composite light signal.

17. The apparatus of claim 16 wherein the sample holder includes a sample cell that comprises work-hardened silver halide.

18. The apparatus of claim 17 wherein the sample cell includes a portion of a conduit that is operable for conveying the sample fluid through the sample region, the portion of the conduit comprising work-hardened silver halide.

19. The apparatus of claim 17 wherein the sample cell comprises a chamber operable for containing the sample fluid, the chamber comprising work-hardened silver halide.

20. The apparatus of claim 19 wherein the sample cell includes a first region that is operable for drawing the sample fluid into the sample holder via capillary action, the first region comprising work-hardened silver halide.

21. A method comprising:

locating a sample fluid in a sample region, the sample fluid including a first chemical constituent and a background solvent, the sample fluid being located by a sample holder that enables the sample fluid to receive mid-infrared radiation and provide a composite light signal;

exposing the sample fluid to mid-infrared radiation;

providing the composite light signal such that it includes spectral information based on each of a first chemical constituent and the background solvent;

distributing the first composite light signal into a plurality of wavelength signals; and

providing a first electrical signal based on the plurality of wavelength signals.

22. The method of claim 21 further comprising generating an output signal that is based on first electrical signal and a first spectral model, wherein the first spectral model is based on the mid-infrared spectral signature of the background solvent.

23. The method of claim 21 further comprising providing the infrared radiation, wherein the mid-infrared radiation is provided by operations comprising:

providing a drive signal to a source, wherein the source is dimensioned and arranged to convert the drive signal into mid-infrared radiation; and

modulating the drive signal such that it comprises a plurality of pulses of electrical energy.

24. The method of claim 21 further comprising providing a sample holder, wherein the sample holder includes a portion of a conduit that conveys the sample fluid to the sample region, the portion of the conduit comprising work-hardened silver halide.

25. The method of claim 21 further comprising providing a sample holder, wherein the sample holder includes a chamber that contains the sample fluid in the sample region, the chamber comprising work-hardened silver halide.

26. The method of claim 21 further comprising providing a sample holder, wherein the sample holder includes a first region that is operable for drawing the sample fluid into the sample holder via capillary action, the first region comprising work-hardened silver halide.

27. The method of claim 21 wherein the sample fluid is exposed to mid-infrared radiation by operations comprising:

providing a drive signal to a source, wherein the source converts the drive signal into mid-infrared radiation; and

conveying mid-infrared radiation from the source to the sample region via a first optical fiber that comprises a first material that is substantially transparent for mid-infrared radiation.

28. The method of claim 27 further comprising providing the first optical fiber such that the first material comprises silver halide.

29. The method of claim 21 wherein the composite light signal is distributed into the plurality of wavelength signals by operations comprising:

coupling the composite light signal into a first optical fiber at the sample region, the first optical fiber comprising a first material that is substantially transparent for mid-infrared radiation, wherein the first optical fiber is optically coupled with a wavelength discriminator operative to spatially disperse the composite light signal into the plurality of wavelength signals; and

emitting the composite light signal from the first optical fiber such that it is received by the wavelength discriminator.

30. The method of claim 29 further comprising providing the first optical fiber such that the first material comprises silver halide.