RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE

• US 20130078673A1
• Filed: 11/02/2012
• Published: 03/28/2013
• Est. Priority Date: 12/18/2007
• Status: Abandoned Application

• Current Assignee: Korea Advanced Institute of Science and Technology
• Original Assignee: Korea Advanced Institute of Science and Technology
• Inventors: LEE, Sang Yup, LEE, Jeong Wook, SONG, Hyohak, CHOI, Sol, KIM, Ji Mahn, PARK, Jin Hwan
• Application Number:
  US13/667,612
• Publication Number:
  US 20130078673A1
• Time in Patent Office: Days
• US Class Current: 435/69.1
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 15/70 Vectors or expression systems specially adapted for E. coli
• CPC Class Codes: C12N 9/1205 Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)
• CPC Class Codes: C12N 9/2408 acting on alpha -1,4-glucosidic bonds
• CPC Class Codes: C12P 1/04 by using bacteria
• CPC Class Codes: C12P 13/08 Lysine; Diaminopimelic acid; Threonine; Valine
• CPC Class Codes: C12P 7/065 with microorganisms other than yeasts
• CPC Class Codes: C12P 7/40 containing a carboxyl group including Peroxycarboxylic acids
• CPC Class Codes: C12P 7/46 Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
• CPC Class Codes: C12P 7/54 Acetic acid vinegar C12J
• CPC Class Codes: C12P 7/56 Lactic acid
• CPC Class Codes: C12Y 207/01004 Fructokinase (2.7.1.4)
• CPC Class Codes: C12Y 207/01069 Protein-Npi-phosphohistidine-sugar phosphotransferase (2.7.1.69), i.e. sucrose phosphotransferase system II
• CPC Class Codes: C12Y 302/01026 Beta-fructofuranosidase (3.2.1.26), i.e. invertase
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.

Claims

1-22. -22. (canceled)

23. A recombinant vector containing a gene (ptsG) encoding a sucrose phosphotransferase and a sacC gene encoding sucrose-6-phosphate hydrolase, wherein the ptsG gene is represented by a base sequence of SEQ ID NO:

2.

24. The recombinant vector according to claim 23, wherein the sacC gene is represented by a base sequence of SEQ ID NO:

4.

25. A recombinant microorganism capable of metabolizing sucrose in which the recombinant vector of claim 23 is introduced into a host cell selected form the group consisting of bacteria, yeast and fungi.

26. A recombinant microorganism capable of metabolizing sucrose in which a gene (ptsG) encoding a sucrose phosphotransferase and a gene (sacC) encoding sucrose-6-phosphate hydrolase is introduced into a chromosomal DNA of a host cell selected form the group consisting of bacteria, yeast and fungi, wherein the ptsG gene is represented by a base sequence of SEQ ID NO:

2.

27. The recombinant microorganism capable of metabolizing sucrose according to claim 26, wherein the sacC gene is represented by a base sequence of SEQ ID NO:

4.

28. A recombinant microorganism capable of metabolizing sucrose in which a gene encoding a β

-fructofuranosidase having an activity to hydrolyze β

-D-fructofuranoside bond to liberate fructose is introduced into a chromosomal DNA of a host cell selected form the group consisting of bacteria, yeast and fungi.

29. A method for producing metabolites, biodegradable polymers or recombinant proteins, the method comprises;

(a) preparing a recombinant microorganism capable of metabolizing sucrose in which the gene represented by SEQ ID NO;

4 is introduced into a chromosomal DNA of a host cell selected from the group consisting of bacteria and fungi,

(b) culturing the recombinant microorganism in a medium containing sucrose as a carbon source.

30. The method according to claim 29, wherein the recombinant microorganism capable of metabolizing sucrose is E. coli.