METHODS FOR OBTAINING A SEQUENCE
First Claim
Patent Images
1. A method for obtaining a sequence, the method comprising:
- determining an amount of amplifiable nucleic acid present in a sample;
partitioning the nucleic acid based upon results of the determining step such that each partitioned portion comprises, on average, a subset of unique sequences;
sequencing the nucleic acid to obtain sequence reads; and
obtaining a sequence from the reads.
3 Assignments
0 Petitions
Accused Products
Abstract
The invention generally relates to methods for obtaining a sequence, such as a consensus sequence or a haplotype sequence. In certain embodiments, methods of the invention involve determining an amount of amplifiable nucleic acid present in a sample, partitioning the nucleic acid based upon results of the determining step such that each partitioned portion includes, on average, a subset of unique sequences, sequencing the nucleic acid to obtain sequence reads, and assembling a consensus sequence from the reads.
-
Citations
19 Claims
-
1. A method for obtaining a sequence, the method comprising:
-
determining an amount of amplifiable nucleic acid present in a sample; partitioning the nucleic acid based upon results of the determining step such that each partitioned portion comprises, on average, a subset of unique sequences; sequencing the nucleic acid to obtain sequence reads; and obtaining a sequence from the reads. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 13)
-
-
14. A method for obtaining a sequence, the method comprising:
-
obtaining nucleic acid; fragmenting the nucleic acid; determining an amount of amplifiable nucleic acid present in a sample; partitioning the fragmented nucleic acids based upon results of the determining step; amplifying the partitioned nucleic acids; attaching bar codes to the amplified nucleic acid; sequencing the nucleic acid to obtain bar coded sequence reads; and obtaining a sequence from the reads. - View Dependent Claims (15, 16, 17, 18, 19)
-
Specification