MALLEABLE HYDROGEL HYBRIDS MADE OF SELF-ASSEMBLED PEPTIDES AND BIOCOMPATIBLE POLYMERS AND USES THEREOF

• US 20130079421A1
• Filed: 06/02/2011
• Published: 03/28/2013
• Est. Priority Date: 06/03/2010
• Status: Active Grant

• Current Assignee: Technology Innovation Momentum Fund (Israel) Limited
• Original Assignee: Ramot at Tel Aviv University Limited (Tel Aviv University)
• Inventors: Aviv, Moran
• Granted Patent: US 9,074,095 B2
• Time in Patent Office: Days
• US Class Current: 514/773
• CPC Class Codes: A61K 2800/10 General cosmetic use
• CPC Class Codes: A61K 38/05 Dipeptides
• CPC Class Codes: A61K 47/26 Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
• CPC Class Codes: A61K 47/36 Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
• CPC Class Codes: A61K 8/042 Gels
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/735 Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
• CPC Class Codes: A61K 8/736 Chitin; Chitosan; Derivatives thereof
• CPC Class Codes: A61K 9/1694 resulting in granules or microspheres of the matrix type containing more than 5% of excipient
• CPC Class Codes: A61L 2300/25 Peptides having up to 20 amino acids in a defined sequence
• CPC Class Codes: A61L 2400/06 Flowable or injectable implant compositions
• CPC Class Codes: A61L 27/20 Polysaccharides
• CPC Class Codes: A61L 27/227 Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
• CPC Class Codes: A61L 27/26 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 27/50 Materials characterised by their function or physical properties , e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
• CPC Class Codes: A61L 27/52 Hydrogels or hydrocolloids
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: C08L 5/08 Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00

Abstract

Hybrid hydrogels formed of a plurality of peptides that are capable of self-assembling into a hydrogel in an aqueous solution and a biocompatible polymer that is characterized by high swelling capability, high elasticity and low mechanical strength are disclosed, with exemplary hybrid hydrogels being formed of a plurality of aromatic dipeptides and hyaluronic acid. The hybrid hydrogels are characterized by controllable mechanical and biological properties which can be adjusted by controlling the concentration ratio of the peptides and the polymer, and which average the mechanical and biological properties of the peptides and the polymer. Processes of preparing the hydrogels and uses thereof in pharmaceutical, cosmetic or cosmeceutic applications such as tissue engineering and/or regeneration are further disclosed.

Claims

1-57. -57. (canceled)

58. A hydrogel comprising a fibrous network of a plurality of peptides and at least one biocompatible polymer, wherein said peptides are capable of self-assembling in an aqueous solution so as to form a hydrogel and wherein said biocompatible polymer features at least one characteristic selected from the group consisting of:

(i) a storage modulus G′

lower than 500 Pa at 10 Hz frequency and at 25°

C.;

(ii) a swelling ratio (Q) higher than 500;

(iii) a viscosity at 0.1 Sec<sup>−

1</sup> shear rate and at 25°

C., lower than 300 Pa·

s; and

(iv) a viscosity recovery after shear of at least 95%.

59. The hydrogel of claim 58, wherein said biocompatible polymer is a polysaccharide.

60. The hydrogel of claim 59, wherein said biocompatible polymer is hyaluronic acid.

61. The hydrogel of claim 59, wherein said biocompatible polymer is a chitosan.

62. The hydrogel of claim 58, wherein each peptide in said plurality of peptides comprises an amino acid sequence not exceeding 6 amino acids in length, whereas said amino acid sequence comprises at least one aromatic amino acid residue.

63. The hydrogel of claim 62, wherein at least one peptide in said plurality of peptides consists essentially of aromatic amino acid residues.

64. The hydrogel of claim 62, wherein each peptide in said plurality of peptides consists essentially of aromatic amino acid residues.

65. The hydrogel of claim 62, wherein at least one peptide in said plurality of peptides is a dipeptide.

66. The hydrogel of claim 62, wherein each peptide in said plurality of peptides is a dipeptide.

67. The hydrogel of claim 66, wherein at least one of said dipeptides is a homodipeptide.

68. The hydrogel of claim 66, wherein each of said dipeptides is a homopeptide.

69. The hydrogel of claim 67, wherein said homodipeptide is selected from the group consisting of phenylalanine-phenylalanine dipeptide, naphthylalanine-naphthylalanine dipeptide, phenanthrenylalanine-phenanthrenylalanine dipeptide, anthracenylalanine-anthracenylalanine dipeptide, [1,10]-phenanthrolinylalanine-[1,10]-phenanthrolinylalanine dipeptide, [2,2′

]bipyridinylalanine-[2,2′

]bipyridinylalanine dipeptide, (pentahalo-phenylalanine)-(pentahalo-phenylalanine) dipeptide, (amino-phenylalanine)-(amino-phenylalanine) dipeptide, (dialkylamino-phenylalanine)-(dialkylamino-phenylalanine) dipeptide, (halophenylalanine)-(halophenylalanine) dipeptide, (alkoxy-phenylalanine)-(alkoxy-phenylalanine) dipeptide, (trihalomethyl-phenylalanine)-(trihalomethyl-phenylalanine) dipeptide, (4-phenyl-phenylalanine)-(4-phenyl-phenylalanine) dipeptide and (nitro-phenylalanine)-(nitro-phenylalanine) dipeptide.

70. The hydrogel of claim 58, wherein at least one peptide in said plurality of peptides is an end-capping modified peptide.

71. The hydrogel of claim 58, wherein each peptide in said plurality of peptides is an end-capping modified peptide.

72. The hydrogel of claim 70, wherein said end capping modified peptide comprises at least one end capping moiety, said end capping moiety being selected from the group consisting of an aromatic end capping moiety and a non-aromatic end-capping moiety.

73. The hydrogel of claim 72, wherein said aromatic end capping moiety is 9-fluorenylmethyloxycarbonyl (Fmoc).

74. The hydrogel of claim 60, wherein each peptide in said plurality of peptides is a homodipeptide.

75. The hydrogel of claim 74, wherein said homodipeptide is phenylalanine-phenylalanine dipeptide.

76. The hydrogel of claim 75, wherein each dipeptide in said plurality of dipeptides is an end-capping modified peptide.

77. The hydrogel of claim 76, wherein each of said end-capping modified dipeptides comprises an aromatic end-capping moiety.

78. The hydrogel of claim 77, wherein said aromatic end capping moiety is 9-fluorenylmethyloxycarbonyl (Fmoc).

79. The hydrogel of claim 58, wherein a total concentration of said plurality of peptides and said polymer ranges from 0.1 weight percent to 5 weight percents of the total weight of the gel.

80. The hydrogel of claim 58, wherein a weight ratio of said plurality of peptides and said polymer ranges from 10:

1 to 1;

10.

81. The hydrogel of claim 58, wherein said fibrous network comprises a plurality of fibrils, whereas an average diameter of said fibrils ranges from about 10 nm to about 100 nm.

82. The hydrogel of claim 58, characterized by at least one of:

a storage modulus G′

to loss modulus G″

ratio that is greater than 4;

a storage modulus G′

higher than 1,000 Pa at 10 Hz frequency and at 25°

C.; and

a storage modulus G′

lower than 100,000 Pa at 10 Hz frequency and at 25°

C.;

83. The hydrogel of claim 58, characterized by a viscosity that ranges from 200 to 2000 Pa·

s at 0.1 Sec<sup>−

1</sup> shear rate, at 25°

C.;

a viscosity recovery after shear of at least 50%, at 0.1 sec<sup>−

1</sup>; and

a swelling ratio (Q) that ranges from 100 to 500.

84. A composition-of-matter comprising the hydrogel of claim 58 and at least one agent being incorporated therein or thereon.

85. A process of preparing the hydrogel of claim 58, the process comprising contacting said plurality of peptides and said biocompatible polymer in an aqueous solution.

86. The process of claim 85, wherein said contacting is effected ex-vivo.

87. The process of claim 85, wherein said contacting is effected in-vivo.

88. A pharmaceutical, cosmetic or cosmeceutical composition comprising the hydrogel of claim 58.

89. A pharmaceutical, cosmetic or cosmeceutical composition comprising the composition-of-matter of claim 84.

90. An article-of-manufacture comprising the hydrogel of claim 58.

91. An article-of-manufacture comprising the composition-of-matter of claim 84.

92. A kit for forming the hydrogel of claim 58, the kit comprising said plurality of peptides and said biocompatible polymer.

93. The kit of claim 92, further comprising instructions for forming the hydrogel by contacting said peptides and said biocompatible polymer with an aqueous solution.

94. The kit of any of claims 92, further comprising an aqueous solution, wherein said peptides and said aqueous solution are individually packaged within the kit.

95. A kit of for forming the composition-of-matter of claim 84, the kit comprising said plurality of peptides, said biocompatible polymer and said active agent.

96. The kit of claim 95, further comprising instructions for forming the hydrogel by contacting said plurality of peptides, said biocompatible polymer and said active agent with an aqueous solution.

97. The kit of claim 96, further comprising an aqueous solution, wherein said plurality of peptides and said aqueous solution are individually packaged within the kit.

98. A method of repairing a damaged tissue, the method comprising contacting the damaged tissue with the hydrogel of claim 58.

99. A method of repairing a damaged tissue, the method comprising contacting the damaged tissue with the composition-of-matter of claim 84.