FLUIDIC FLOW CYTOMETRY DEVICES AND METHODS
First Claim
1. A flow cytometry device, comprising:
- a channel capable of conducting a fluid containing at least one particle, the channel further capable of allowing light be transmitted to and from the channel;
a lens positioned between the channel and a color filter and adapted to direct at least a portion of light transmitted from the channel to the color filter, the color filter comprising a plurality of zones, each zone adapted to allow transmission of only a particular spectral range of light; and
a detector configured to receive the light that is transmitted through the color filter.
4 Assignments
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Accused Products
Abstract
Devices, systems and methods facilitate analyzing, identifying and sorting particles in fluids, including cytometry devices and techniques. The described techniques can be used in a variety of applications such as in chemical or biological testing and diagnostic measurements. One exemplary flow cytometry device includes a channel that is capable of conducting a fluid containing at least one particle and also capable of allowing light be transmitted to and from the channel. The flow cytometry device also includes a lens that is positioned between the channel and a color filter. The lens directs at least a portion of light transmitted from the channel to the color filter. The color filter includes a plurality of zones, where each zone is adapted to allow transmission of only a particular spectral range of light. The flow cytometry device further includes a detector configured to receive the light that is transmitted through the color filter.
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Citations
33 Claims
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1. A flow cytometry device, comprising:
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a channel capable of conducting a fluid containing at least one particle, the channel further capable of allowing light be transmitted to and from the channel; a lens positioned between the channel and a color filter and adapted to direct at least a portion of light transmitted from the channel to the color filter, the color filter comprising a plurality of zones, each zone adapted to allow transmission of only a particular spectral range of light; and a detector configured to receive the light that is transmitted through the color filter. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A flow cytometry device, comprising:
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a fluidic channel capable of conducting a fluid containing at least one particle, the channel further capable of allowing light be transmitted to and from the fluidic channel; a lens positioned between the fluidic channel and a diffractive component and adapted to direct at least a portion of light transmitted from the fluidic channel to the diffractive component; an aperture positioned between the diffractive component and a detector and adapted to allow a particular spectral range of light received from the diffractive component to reach the detector based upon the position(s) of the at least one particle within the fluidic channel. - View Dependent Claims (8)
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9. An imaging device, comprising:
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a stage configured to move in one or more dimensions and configured to receive an object for imaging, wherein at least a portion of the object is capable of transmitting light; and a lens positioned between the stage and an image sensor and adapted to direct the light emitted by the at least a portion of the object to the image sensor, wherein the image sensor comprises a plurality of light sensing elements arranged as a plurality of sensing element subsets, each sensing element subset being responsive to a particular spectral range of light incident thereupon, and wherein the image sensor is aligned with the lens and the object so as to receive, at a first subset of the sensing elements, a first spectral range of light emitted by the object when the object is at a first position, and to receive a second spectral range of light emitted by the object at a second subset of sensing elements when the object is at a second position. - View Dependent Claims (10, 11, 12)
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13. A method, comprising:
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receiving data corresponding to a particular spectral range of light emitted by a particle in a flow cytometry device; processing the received data to enhance signal-to-noise ratio (SNR) of the received data; determining particle velocity from the processed data with enhanced SNR; determining one or more peak parameters for one or more peaks of the processed data with enhanced SNR; mapping colors in accordance with placement of the peak parameters and/or ratios of the peak parameters; and determining color of the light emitted by the particle in accordance with the mapping. - View Dependent Claims (14)
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15. A method for identifying one or more particles by flow cytometry, comprising:
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flowing one or more particles through a channel of a flow cytometry device; causing the one or more particles to emit light; receiving light emitted by the one or more particles after transmission through a color filter, wherein the color filter comprises a plurality of zones and each zone is configured to allow transmission of only a particular spectral range of light incident thereupon, and wherein light emitted by each particle at different positions within the channel is incident upon different zones of the color filter; producing data associated with the received light; and processing the data to identify the one or more particles. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22)
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23. A method for identifying a particle by an imaging device, comprising:
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causing a particle within a sample affixed to a movable stage to emit light when the movable stage is at a first position; receiving light emitted by the particle at a first section of the imaging device, the first section being responsive to a first spectral range of light incident thereupon; moving the stage to one or more additional positions; receiving light emitted from the particle when the stage is positioned at each of the one or more additional positions at corresponding one or more additional sections of the imaging device, wherein each additional section of the imaging device is responsive to a particular spectral range of light incident thereupon; processing data corresponding to the light received at the first section and at the one or more additional sections of the imaging device to identify the particle. - View Dependent Claims (24, 25, 26, 27, 28)
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29. A method, comprising:
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flowing at least one particle through a fluidic channel of a flow cytometry device; causing the at least one particle to emit light; using a lens to direct the emitted light to a diffractive component, the diffractive component directing a portion of the emitted light to an aperture; receiving, at a detector, the light is propagated through the aperture, wherein the received light at the detector consists of a particular spectral range dependent at least in-part on position(s) of the at least one particle within the fluidic channel; processing data corresponding to the light received at the detector to identify the one or more particles. - View Dependent Claims (30, 31, 32, 33)
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Specification