Disposable Fluid Circuits And Methods For Cell Washing With On-Line Dilution Of Cell Feed
First Claim
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1. A system for the treatment of biological fluid including biological cells comprising:
- (a) a reusable cell processing apparatus comprising a separator element for receiving a separation device and effecting the separation of said biological fluid into two or more components and a programmable microprocessor programmed to process biological fluid through a fluid circuit, said microprocessor being programmed to instruct said system to deliver a pre-selected volume of diluent to a source of biological fluid; and
(b) a disposable fluid circuit comprising at least one membrane separation device in fluid communication with a first product container and an access device for sterile connection to a source of biological fluid.
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Abstract
Systems and methods for the washing and processsing of biological fluid/biological cells are disclosed. The systems and methods prevent inadvertent target cell loss by monitoring pressure and providing for the dilution of the cell feed.
42 Citations
16 Claims
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1. A system for the treatment of biological fluid including biological cells comprising:
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(a) a reusable cell processing apparatus comprising a separator element for receiving a separation device and effecting the separation of said biological fluid into two or more components and a programmable microprocessor programmed to process biological fluid through a fluid circuit, said microprocessor being programmed to instruct said system to deliver a pre-selected volume of diluent to a source of biological fluid; and (b) a disposable fluid circuit comprising at least one membrane separation device in fluid communication with a first product container and an access device for sterile connection to a source of biological fluid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for washing biological cells comprising:
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obtaining a separator comprising a relatively rotatable cylindrical housing and an internal member, wherein said cylindrical housing has an interior surface and said internal member has an exterior surface, said surfaces defining a gap therebetween, wherein at least one of said surfaces includes a porous membrane; drawing biological cells from a container in flow communication with said separator; diluting said biological cells; introducing said diluted cells into said gap of said separator; rotating at least one or both of said housing and said internal member; separating said cells from said liquid medium; concentrating said cells; removing at least some of said concentrated cells from said separator through a first outlet; removing at least some of said separated liquid medium from said separator through a second outlet; monitoring the pressure; and optionally adjusting the dilution of said cells based on said pressure monitoring. - View Dependent Claims (11, 12, 13, 14, 15, 16)
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Specification