Casein Derived Peptides And Uses Thereof

• US 20130096073A1
• Filed: 09/05/2012
• Published: 04/18/2013
• Est. Priority Date: 03/01/2000
• Status: Active Grant

• Current Assignee: Zvi Sidelman
• Original Assignee: Zvi Sidelman
• Inventors: SIDELMAN, Zvi
• Granted Patent: US 8,735,348 B2
• Time in Patent Office: Days
• US Class Current: 514/21.6
• CPC Class Codes: A61K 38/08 Peptides having 5 to 11 amino acids A61K38/043 - A61K38/046 take precedence
• CPC Class Codes: A61K 38/1709 from mammals
• CPC Class Codes: A61P 3/00 Drugs for disorders of the metabolism of the blood or the extracellular fluid A61P7/00
• CPC Class Codes: A61P 3/06 Antihyperlipidemics
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 3/12 for electrolyte homeostasis
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 31/18 for HIV
• CPC Class Codes: A61P 37/00 Drugs for immunological or allergic disorders
• CPC Class Codes: A61P 37/02 Immunomodulators
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid
• CPC Class Codes: A61P 7/04 Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
• CPC Class Codes: A61P 7/06 Antianaemics
• CPC Class Codes: C07K 14/4732 Casein in foodstuffs A23J
• CPC Class Codes: C12P 21/06 produced by the hydrolysis of a peptide bond, e.g. hydrolysate products preparing foodstuffs by protein hydrolysis A23J3/00

Abstract

Biologically active peptides that are derived from or are similar to sequences identical with the N-terminus of the αS1 fraction of milk casein. These peptides are capable of stimulating and enhancing immune response, protecting against viral infection, normalizing serum cholesterol levels, and stimulating hematopoiesis. The casein-derived peptides are non-toxic and can be used to treat and prevent immune pathologies, hypercholesterolemia, hematological disorders and viral-related diseases, alone or in combination with other peptides or blood cell stimulating factors.

Claims

1. A method of treating AIDS or infection by HIV, the method comprising administering to a subject in need thereof a therapeutically effective amount of peptides derived from natural alphaS1, alphaS2, beta and kappa casein, and is prepared according to a preparation method comprising a) low-temperature proteolytic digestion of said natural alphaS1, alphaS2, beta and kappa casein in solution to obtain a casein proteolytic hydrolysate;

b) cooling said casein proteolytic hydrolysate so as to inactivate said proteolytic enzymes;

c) adjusting the pH of said casein protein hydrolysate to an acid pH;

d) centrifuging said acidic casein protein hydrolysate, collecting the supernatant, filtering said supernatant and further acidifying said supernatant so as to precipitate proteins derived from natural casein;

e) separating and collecting said precipitate;

f) adjusting the pH of said precipitate to an alkaline pH so as to irreversibly inactivate said proteolytic enzymes; and

g) adjusting the pH of said precipitate to below pH 9.

2. The method of claim 1, wherein further acidifying said filtrate of stage d comprises adding acid to about at least 10% (v/v) acid.

3. The method of claim 2, wherein said adjusting said pH of stage f comprises increasing a pH of said precipitate to pH 9-13.

4. A method of treating or preventing infectious disease or condition is selected from the group consisting of AIDS, and infection by HIV, the method comprising administering to a subject in need thereof a therapeutically effective amount of peptides derived from natural alphaS1, alphaS2, beta and kappa casein, wherein said peptides are prepared according to a preparation method comprising proteolytic digestion of said natural alphaS1, alphaS2, beta and kappa casein in solution to form a digested material solution;

and centrifugation of said digested material solution to obtain said peptides.

5. A method of treating AIDS or infection by HIV, the method comprising administering to a subject in need thereof a therapeutically effective amount of a peptide consisting of the amino acid sequence set forth in any one of SEQ ID NOs 4, 5 and 10.