DETECTION METHOD AND APPARATUS OF ACTIVATED NEUTROPHILS
First Claim
1. A detection method of activated neutrophils, comprising:
- (a) preparing a measurement sample in which erythrocytes in a biotic sample are hemolyzed and nucleic acid of leukocytes is stained by a nucleic acid staining fluorochrome;
(b) irradiating the prepared measurement sample with light and measuring scattered light intensity and fluorescence intensity generated from particles in the measurement sample;
(c) classifying the leukocytes in the biotic sample into at least a cluster containing neutrophils and a cluster containing eosinophils based on the acquired scattered light intensity and fluorescence intensity; and
(d) detecting particles present between the cluster containing the neutrophils and the cluster containing the eosinophils as activated neutrophils.
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Accused Products
Abstract
To provide a detection method of activated neutrophils enabling the classification of normal leukocytes and the detection of activated neutrophils, a measurement sample in which erythrocytes in a biotic sample are hemolyzed and nucleic acid of leukocytes is stained by a nucleic acid staining fluorochrome is prepared, the sample is irradiated with light, and leukocytes in the biotic sample are classified into at least a cluster containing neutrophils and a cluster containing eosinophils based on scattered light intensity and fluorescence intensity obtained by measuring the scattered light intensity and fluorescence intensity generated from particles in the sample to detect particles present between the cluster containing neutrophils and the cluster containing eosinophils as activated neutrophils.
5 Citations
16 Claims
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1. A detection method of activated neutrophils, comprising:
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(a) preparing a measurement sample in which erythrocytes in a biotic sample are hemolyzed and nucleic acid of leukocytes is stained by a nucleic acid staining fluorochrome; (b) irradiating the prepared measurement sample with light and measuring scattered light intensity and fluorescence intensity generated from particles in the measurement sample; (c) classifying the leukocytes in the biotic sample into at least a cluster containing neutrophils and a cluster containing eosinophils based on the acquired scattered light intensity and fluorescence intensity; and (d) detecting particles present between the cluster containing the neutrophils and the cluster containing the eosinophils as activated neutrophils. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A detection apparatus of activated neutrophils, comprising:
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a sample preparation unit that prepares a measurement sample by mixing a biotic sample, a fluorochrome to stain nucleic acid, and a hemolyzing agent containing a surfactant that hemolyzes erythrocytes and damages cell membranes of leukocytes to such an extent that the fluorochrome can pass through; a measuring unit that irradiates the prepared measurement sample with light and measures scattered light intensity and fluorescence intensity generated from particles in the measurement sample; and a controller configured for performing operations, comprising; (a) classifying the leukocytes in the biotic sample into at least a cluster containing neutrophils and a cluster containing eosinophils based on the acquired scattered light intensity and fluorescence intensity; and (b) detecting particles present between the cluster containing the neutrophils and the cluster containing the eosinophils as activated neutrophils. - View Dependent Claims (11, 12, 13, 14, 15, 16)
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Specification