METHODS OF CELL CULTURE FOR ADOPTIVE CELL THERAPY
First Claim
1. An improved method of producing cells comprising:
- growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with a medium volume to surface area ratio of up to 1 ml/cm2 if a growth surface is not comprised of gas permeable material and up to 2 ml/cm2 if the growth surface is comprised of gas permeable material;
wherein said desired cells are at a surface density of less than 0.5×
106 cells/cm2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×
105 cells/cm2.
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Abstract
An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5×106 cells/cm2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×105 cells/cm2.
93 Citations
2 Claims
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1. An improved method of producing cells comprising:
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growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with a medium volume to surface area ratio of up to 1 ml/cm2 if a growth surface is not comprised of gas permeable material and up to 2 ml/cm2 if the growth surface is comprised of gas permeable material; wherein said desired cells are at a surface density of less than 0.5×
106 cells/cm2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×
105 cells/cm2.
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2. A static gas permeable cell culture and cell recovery device comprising:
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a growth surface comprised of gas permeable material, wherein said growth surface residing in a horizontal position when the device is in operation, a medium removal conduit including a medium removal opening, a cell removal conduit including a cell removal opening, an internal volume, and an upper confine bounding the uppermost location of said internal volume, wherein the distance from the upper confine to the growth surface being a potential medium height and the distance from the upper confine to the growth surface being beyond 2.0 cm, and wherein the distance said medium removal conduit resides above said growth surface at least 0.2 cm above said growth surface when said growth surface resides in a horizontal position, and no more than beyond 50% of said potential medium height.
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Specification