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METHOD TO MEASURE THE METABOLIC RATE OR RATE OF GLUCOSE CONSUMPTION OF CELLS OR TISSUES WITH HIGH SPATIOTEMPORAL RESOLUTION USING A GLUCOSE NANOSENSOR

  • US 20130111611A1
  • Filed: 06/30/2010
  • Published: 05/02/2013
  • Est. Priority Date: 06/30/2010
  • Status: Abandoned Application
First Claim
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1. A method for the measurement of glucose metabolic rate with high temporal resolution, comprising the steps of:

  • a. providing single cells or cell populations, cells in suspension or adherent, in a cell culture, a tissue culture, a mixed cell culture, a tissue explant, or in animal tissues in vivo for the measurement of glucose metabolic rate;

    b. expressing a glucose sensor in individual cells;

    c. calibrating the sensor in controlled conditions;

    d. disrupting the steady-state of glucose entering the cell;

    e. recording the output from the sensor and calculating the corresponding glucose concentration at different times; and

    f. determining the glucose metabolic rate.

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