Cellular Libraries of Peptide Sequences (CLiPS) and Methods of Using the Same
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Abstract
The present invention provides compositions including peptide display scaffolds that present at least one candidate peptide and at least one detectable moiety in at least one of the N-terminal and C-terminal candidate peptide presenting domains that when expressed in a cell are accessible at a surface of the cell outermembrane. In addition, the present invention also provides kits and methods for screening a library of cells presenting the candidate peptides in peptide display scaffolds to identify a ligand for an enzyme.
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Citations
66 Claims
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1-46. -46. (canceled)
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47. A cell comprising a peptide display scaffold disposed in an outer membrane of the cell, the peptide display scaffold comprising a fusion protein comprising the formula:
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[D1-C1]-TM-[D2]wherein TM is a circularly permuted bacterial outer membrane protein; C1 comprises a candidate peptide; and D1 and D2 are first and second detectable moieties, wherein D1 and D2 are not the same and wherein D1-C1 and D2 are exposed at an extracellular surface of the cell outer membrane and one or both of the N- and C-termini of the fusion protein are exposed at the extracellular surface of the cell outer membrane. - View Dependent Claims (48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66)
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Specification