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SAMPLE-TO-ANSWER MICROFLUIDIC CARTRIDGE

  • US 20130130262A1
  • Filed: 01/28/2011
  • Published: 05/23/2013
  • Est. Priority Date: 01/29/2010
  • Status: Active Grant
First Claim
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1. A microfluidic cartridge for assay of a target analyte or analytes in a biological sample, the microfluidic cartridge comprising:

  • a) a plastic, thermally insulative cartridge body enclosing;

    i) a hydraulic works comprising an upstream sample inlet, one or more on-board liquid or dry reagents for an assay, and a wettable downstream microfluidic subcircuit comprising a channels and chamber fluidly connected to said upstream inlet and vented at a downstream vent; and

    ii) a pneumatic works comprising an inlet or inlets for receiving pneumatic pressure and a pneumatic subcircuit comprising a channel and chamber for conveying said pneumatic pressure therefrom; and

    b) at least one pneumohydraulic diaphragm configured for interfacing a hydraulic chamber of said wettable microfluidic subcircuit with a pneumatic chamber of said pneumatic subcircuit, said pneumohydraulic diaphragm having a first side facing said hydraulic chamber and a second side facing said pneumatic chamber;

    whereinsaid at least one pneumohydraulic diaphragm is;

    A) an elastic, energy-storing pneumohydraulic diaphragm configured for passively storing a liquid volume under a hydraulic pressure and releasing said liquid volume during wetout of a downstream channel or chamber of said wettable microfluidic subcircuit;

    B) a duplexedly layered pneumohydraulic diaphragm having a liquid center for storing and releasing a liquid reagent;

    C) a pneumohydraulic diaphragm configured for eliminating headspace from a hydraulic chamber during wetout;

    orD) a pair of pneumohydraulic diaphragms comprising a first pneumohydraulic diaphragm interfacing a first hydraulic chamber with valved inlet and a second pneumohydraulic diaphragm interfacing a second hydraulic chamber with valved outlet, wherein said pair of pneumohydraulic diaphragms comprise an elevated intercommunicating channel between said first and second hydraulic chambers, wherein said pair of pneumohydraulic diaphragms is configured for reciprocally exchanging fluid through said channel by applying opposing pressure differentials across said first and second pneumohydraulic diaphragms; and

    wherein said hydraulic chambers and diaphragms are configured for preventing or reducing bubble entrainment or reagent washout during wetout, fill, pumping or rehydration steps of the assay.

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