Expression of Proteins in Plants
First Claim
1. A method of producing an influenza virus H5 polypeptide in a plant comprising the steps of:
- i) cloning an influenza virus H5 gene or nucleic acid encoding a functional equivalent thereof into a vector adapted to target components present in the plant, wherein the plant components are selected from the group consisting of endoplasmic reticuli and apoplasts;
ii) infiltrating at least a portion of the plant with the vector or transforming plant tissue with the vector so as to either transiently express the influenza virus H5 polypeptide, and/or to create a transgenic plant; and
iii) recovering the influenza virus H5 polypeptide expressed by the plant.
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Abstract
The invention relates to a method of producing an influenza virus H5 polypeptide in a plant comprising the steps of cloning an influenza H5 gene or nucleic acid encoding its functional equivalent into a vector adapted to target components present in the plant, infiltrating at least a portion of the plant with the vector or transforming plant tissue with the vector so as to transiently express the influenza virus H5 polypeptide, and/or to create a transgenic plant; and recover the influenza virus H5 polypeptide expressed by the plant. The invention further relates to vectors, transgenic plants or parts thereof and the progeny of such plants used in or which come about as a result of the method.
5 Citations
15 Claims
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1. A method of producing an influenza virus H5 polypeptide in a plant comprising the steps of:
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i) cloning an influenza virus H5 gene or nucleic acid encoding a functional equivalent thereof into a vector adapted to target components present in the plant, wherein the plant components are selected from the group consisting of endoplasmic reticuli and apoplasts; ii) infiltrating at least a portion of the plant with the vector or transforming plant tissue with the vector so as to either transiently express the influenza virus H5 polypeptide, and/or to create a transgenic plant; and iii) recovering the influenza virus H5 polypeptide expressed by the plant. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 15)
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13. Use of a vector into which an influenza virus H5 gene has been cloned, which vector is adapted to target endoplasmic reticuli or apoplast components present in a plant so as to either transiently express the influenza virus H5 polypeptide in the cytoplasm and then import the influenza virus H5 polypeptide into the plant components, and/or to create a transgenic plant where the influenza virus H5 polypeptide is expressed in the cytoplasm and then imported into the plant components.
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14. A vector into which an influenza virus H5 gene has been cloned, which vector is adapted to target endoplasmic reticuli or apoplast components present in a plant so as to either transiently express the influenza virus H5 polypeptide in the cytoplasm and then import the influenza virus H5 polypeptide into the plant components, and/or to create a transgenic plant where the influenza virus H5 polypeptide is expressed in the cytoplasm and then imported into the plant components.
Specification