COMPOSITIONS AND METHODS FOR SIMULTANEOUS DETECTION OF VOLATILE SULFUR COMPOUNDS AND POLYAMINES

• US 20130130397A1
• Filed: 01/17/2013
• Published: 05/23/2013
• Est. Priority Date: 10/18/2002
• Status: Abandoned Application

• Current Assignee: ALT Bioscience LLC
• Original Assignee: ALT Bioscience LLC
• Inventors: Pendergrass, James C., Haley, Boyd E.
• Application Number:
  US13/744,059
• Publication Number:
  US 20130130397A1
• Time in Patent Office: Days
• US Class Current: 436/119
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72
• CPC Class Codes: Y10T 436/173845 Amine and quaternary ammonium
• CPC Class Codes: Y10T 436/18 Sulfur containing

Abstract

Disclosed are compositions and methods useful for the rapid and facile simultaneous detection of malodorous bacterial metabolites in samples of expired breath and other fluids. The invention enables estimation, by simple visual inspection and comparison against standards, of the concentration of polyamines and volatile sulfur compounds in the micromolar to millimolar range.

Claims

1. A matrix impregnated with a chromogenic composition useful for the detection of volatile sulfur compounds (VSCs), comprising DTNB, a buffer, and highly purified water as solvent, wherein the matrix is selected from matrices that are inert.

2. A matrix of claim 1, wherein the matrix is a filter paper disk.

3. A matrix of claim 1, wherein the matrix is cotton.

4. A matrix of claim 1, wherein the matrix is a polysaccharide.

5. The matrix of claim 1, wherein the composition is stable for 12 months at room temperature.