METHODS AND COMPOSITIONS FOR MODIFICATION OF THE HPRT LOCUS
First Claim
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1. A non-naturally occurring fusion protein comprising a zinc finger protein that binds to an endogenous hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene and a cleavage domain, wherein the fusion protein modifies the endogenous HPRT gene.
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Abstract
Nucleases and methods of using these nucleases for modification of an HPRT locus and for increasing the frequency of gene modification at a targeted locus and clones and for generating animals.
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Citations
26 Claims
- 1. A non-naturally occurring fusion protein comprising a zinc finger protein that binds to an endogenous hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene and a cleavage domain, wherein the fusion protein modifies the endogenous HPRT gene.
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2. The fusion protein of claim 2, wherein the zinc finger protein comprises 4, 5 or 6 zinc finger domains comprising a recognition helix region, wherein the zinc finger proteins comprise the recognition helix regions in the order shown in a single row of Table 1.
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