Anti-Interferent Barrier Layers for Non-Invasive Transdermal Sampling and Analysis Device
First Claim
1. A transdermal sampling and analysis device comprising:
- a substrate having a first side;
at least one disruptor mounted on the first side of the substrate, wherein the at least one disruptor is configured to generate a localized heat capable of altering permeability characteristics of a stratum corneum layer of skin of an organism;
a reservoir configured to collect and contain a biological sample; and
a biological sensing element comprising at least two sensing electrodes configured, to determine levels of an analyte in the biological sample, wherein;
a surface of at least one of the sensing electrodes is coated with a sensing layer having a first charge type; and
the sensing layer is covered with an anti-interferent barrier layer having at least a second charge type, wherein the first charge type is an opposite charge type from the second charge type.
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Abstract
A system and methods are provided for reducing electrochemical interference in a transdermal sampling and analysis device. A one-step transdermal glucose biosensor may calculate glucose concentrations that are artificially high compared to traditional home blood glucose sensors due to interference, which may be mitigated by forming an anti-interferent barrier layer over a sensing element. The anti-interferent barrier layer may be formed over a sensing layer and may possess a charge type which repels interferent molecules having the same charge type from interacting with the sensing layer disposed below the anti-interferent barrier layer.
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Citations
38 Claims
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1. A transdermal sampling and analysis device comprising:
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a substrate having a first side; at least one disruptor mounted on the first side of the substrate, wherein the at least one disruptor is configured to generate a localized heat capable of altering permeability characteristics of a stratum corneum layer of skin of an organism; a reservoir configured to collect and contain a biological sample; and a biological sensing element comprising at least two sensing electrodes configured, to determine levels of an analyte in the biological sample, wherein; a surface of at least one of the sensing electrodes is coated with a sensing layer having a first charge type; and the sensing layer is covered with an anti-interferent barrier layer having at least a second charge type, wherein the first charge type is an opposite charge type from the second charge type. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14)
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12. The transdermal sampling and analysis device of claim S, wherein the first anti-interferent barrier layer comprises an alginate monolayer;
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the second anti-interferent barrier layer comprises chitosan.
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15. A transdermal sampling and analysis device comprising:
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a substrate having a first side; at least one disruptor mounted on the first side of the substrate, wherein the at least one disruptor is configured to generate a localized heat capable of altering permeability characteristics of a stratum corneum layer of skin of an organism; a reservoir configured to collect, and contain a biological sample; and a biological sensing element comprising at least two sensing electrodes configured to determine levels of an analyte in the biological sample, wherein; a surface of at least one of the sensing electrodes is coated with a sensing layer; and the sensing layer is covered with an anti-interferent barrier layer, wherein molecules of the anti-interferent barrier layer are conjugated to molecules of the sensing layer. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A transdermal sampling and analysis device comprising:
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a substrate having a first side; at least one disruptor mounted on the first side of the substrate, wherein the at least one disruptor is configured to generate a localized heat, capable of altering permeability characteristics of a stratum corneum layer of skin of an organism; a reservoir configured to collect and contain a biological sample; and a biological sensing element comprising at least two sensing electrodes configured to determine levels of an analyte in the biological sample, wherein; a surface of at least one of the sensing electrodes is coated with a sensing layer, wherein the sensing layer has an electron mediator; and the sensing layer is covered with an anti-interferent barrier layer, wherein the anti-interferent barrier layer has a charge type to repel a charged biological reducing species in the biological sample from interacting with the electron mediator. - View Dependent Claims (28, 29, 30, 31, 32, 33, 38)
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34. A method of preventing interference from a charged biological reducing species in a transdermal biosensor device, comprising:
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creating a solid state sensor wherein an enzyme and an electron mediator are immobilized by a polymer to form a sensing layer, wherein the polymer is anchored to a sensing electrode; and applying an anti-interferent barrier layer atop the sensing layer, wherein charge type repulsion between the anti-interferent barrier layer and the charged biological reducing species prevents interaction between the charged biological reducing species and the electron mediator. - View Dependent Claims (35, 36, 37)
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Specification