METHODS FOR OBTAINING STEM CELLS
First Claim
Patent Images
1. A method for obtaining mammalian stem cells comprising the following steps:
- a) harvesting a tissue from a mammalian cadaver stored at 1-6°
C., the harvesting being performed on a part of the cadaver'"'"'s body in which stem cells are usually present in a living counterpart; and
b) extracting mononuclear cells from the harvested tissue.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to methods for obtaining stem cells from mammalian cadavers, methods for obtaining or purifying stem cells from a sample likely to contain non-stem cells, methods of regeneration of injured tissues and methods of treatment.
-
Citations
30 Claims
-
1. A method for obtaining mammalian stem cells comprising the following steps:
-
a) harvesting a tissue from a mammalian cadaver stored at 1-6°
C., the harvesting being performed on a part of the cadaver'"'"'s body in which stem cells are usually present in a living counterpart; andb) extracting mononuclear cells from the harvested tissue. - View Dependent Claims (2, 4, 5, 6, 7, 8, 9, 10)
-
-
3. A method for obtaining mammalian stem cells expressing a transgene of interest, comprising the following steps:
-
a) harvesting a tissue from a mammalian cadaver stored at 1-6°
C., the harvesting being performed on a part of the cadaver'"'"'s body in which stem cells are usually present in a living counterpart; andb) extracting mononuclear cells from the harvested tissue; b′
) optionally culturing the extracted mononuclear cells of step b); andc) transforming or transfecting with a vector, especially a vector of expression, or transducing with a virus vector, the mononuclear cells of step b), or step b′
) where appropriate, said vector comprising at least one polynucleotide sequence of interest.
-
-
11. A method for obtaining stem cells comprising the following steps:
-
a) maintaining a biological material which usually comprises stem cells at an oxygen concentration equal to or less than 0.1%; and b) selecting viable cell, wherein viable cells are stem cells. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
-
-
12. A method for obtaining mammalian stem cells expressing a transgene of interest, comprising the following steps:
-
a) maintaining a biological material which usually comprises stem cells at an oxygen concentration equal to or less than 0.1%; and b) selecting viable cell, wherein viable cells are stem cells. c) transforming or transfecting with a vector, especially a vector of expression, or transducing with a virus vector, the viable cells of step b), said vector comprising at least one polynucleotide sequence of interest.
-
- 23. A method for culturing mammalian stem cells comprising using an anaerobic cell culture system for culturing mammalian stem cells at an oxygen concentration equal to or less than 0.1%.
-
26. A method for selecting stem cells comprising using an anaerobic cell culture system for selecting for stem cells by maintaining at an oxygen concentration equal to or less than 0.1% a biological sample comprising stem cells and non-stem cells.
- 27. An isolated muscle stem cell characterized in that it does not express myogenin gene (Myogenin−
Specification