METHODS FOR MULTIPLEXED SELECTION OF FUNCTIONAL LIGANDS
First Claim
1. A method of selecting aptamers comprising:
- contacting a plurality of identifiers each of which comprising an oligonucleotide comprising a unique identity sequence denoting a predetermined position on a substrate with a corresponding plurality of target spots disposed at predetermined positions on said substrate, wherein each of said target spots comprising at least one potential aptamer bound to at least one target molecule; and
attaching any of said plurality of identifiers to any of said at least one aptamers bound at one of said target spots by hybridizing, each of said oligonucleotides comprising a first constant region which hybridizes to a second constant region on each of said aptamers;
wherein each of said unique identity sequences of said plurality of identifiers correlate to one of said predetermined positions on said substrate.
0 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to methods for generating functional biomolecules. In one exemplary aspect of the invention, generation of functional biomolecules may be performed against multiple targets simultaneously within a single system. In general, a plurality of targets may be disposed within in a single reaction volume and a library of biomolecules, such as a nucleic acid library, may be applied to the reaction volume. The members of the library that do not bind to any of the plurality of targets under given conditions may then be partitioned. The remaining members of the library may then be marked and/or tagged, such as to identify the particular target or targets to which the member of the library binds. The binding members of the library may then be isolated and, by virtue of the marking or tagging, be matched to a particular target or targets.
-
Citations
20 Claims
-
1. A method of selecting aptamers comprising:
-
contacting a plurality of identifiers each of which comprising an oligonucleotide comprising a unique identity sequence denoting a predetermined position on a substrate with a corresponding plurality of target spots disposed at predetermined positions on said substrate, wherein each of said target spots comprising at least one potential aptamer bound to at least one target molecule; and attaching any of said plurality of identifiers to any of said at least one aptamers bound at one of said target spots by hybridizing, each of said oligonucleotides comprising a first constant region which hybridizes to a second constant region on each of said aptamers; wherein each of said unique identity sequences of said plurality of identifiers correlate to one of said predetermined positions on said substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
-
-
10. A method of selecting aptamers from a library of oligonucleotides comprising:
-
contacting a plurality of identifiers each of which comprising an oligonucleotide comprising a unique identity sequence denoting a predetermined position on a substrate with a corresponding plurality of target spots disposed at predetermined positions on said substrate, each of said target spots comprising least one target molecule contacted with members of a library of oligonucleotides and washed to remove non-binders; and attaching any of said plurality of identifiers to any of said members of said library of oligonucleotides bound at one of said target spots by hybridizing, each of said identifiers comprising a first constant region which hybridizes to a second constant region on each of said members of said library of oligonucleotides; wherein each of said unique identity sequences of said plurality of identifiers correlate to one of said predetermined positions on said substrate. - View Dependent Claims (11, 12, 13, 14, 15, 16)
-
-
17. A method of selecting aptamers comprising:
-
contacting an identifier comprising an oligonucleotide comprising a unique identity sequence denoting a predetermined position on a substrate with a corresponding target spot disposed at said predetermined position on said substrate, said target spot comprising an aptamer bound to a target molecule; attaching said identifier to said aptamer bound at said target spot by hybridizing, said oligonucleotide comprising a first constant region which hybridizes to a second constant region on said aptamer; and performing a nucleic acid extension reaction on said hybridized identifier and said aptamer to produce an extension product comprising said identity sequence and a complementary sequence to said aptamer, or their complements; wherein each of said unique identity sequence correlates to said predetermined position on said substrate. - View Dependent Claims (18, 19, 20)
-
Specification