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REAGENTS AND METHODS RELATING TO DNA ASSAYS USING AMPLICON PROBES ON ENCODED PARTICLES

  • US 20130157899A1
  • Filed: 02/21/2013
  • Published: 06/20/2013
  • Est. Priority Date: 12/05/2007
  • Status: Active Grant
First Claim
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1. A method of preparing an encoded bead set for assaying DNA, comprising:

  • a) performing a first amplification reaction using a DNA template and first reaction oligonucleotide primers, each of the plurality of first reaction primers comprising a variable non-specific degenerate DNA sequence and a contiguous constant DNA sequence, to yield a first reaction product comprising first amplicons, wherein each individual first amplicon comprises a DNA sequence identical to a random portion of the DNA template and a DNA sequence identical to the constant DNA sequence of the first reaction primers;

    b) performing a second amplification reaction using at least a portion of the first amplicons as template DNA and second reaction oligonucleotide primers, the second reaction oligonucleotide primers comprising the constant DNA sequence of the first reaction primers, to yield a second reaction product comprising second amplicons, wherein each individual second amplicon comprises a DNA sequence identical to a random portion of the DNA template and a DNA sequence identical to the constant DNA sequence of the first reaction primers;

    c) binding the second amplicons to a first plurality of encoded particles, yielding an encoded particle set for assaying DNA.

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