ASSAYS FOR HUMAN NT-PRO B-TYPE NATRIURETIC PEPTIDE, HUMAN PRO B-TYPE NATRIURETIC PEPTIDE AND HUMAN B-TYPE NATRIURETIC PEPTIDE

• US 20130164767A1
• Filed: 02/27/2013
• Published: 06/27/2013
• Est. Priority Date: 11/21/2009
• Status: Abandoned Application

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Konrath, John G., Moore, Jeff A.
• Application Number:
  US13/778,825
• Publication Number:
  US 20130164767A1
• Time in Patent Office: Days
• US Class Current: 435/7.92
• CPC Class Codes: G01N 2333/58 Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Brain natriuretic peptide [BNP, proBNP]; Cardionatrin; Cardiodilatin
• CPC Class Codes: G01N 33/6887 from muscle, cartilage or connective tissue
• CPC Class Codes: G01N 33/74 involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors

Abstract

The present disclosure relates to assays for detecting and/or quantifying the amount of human NT-pro B-type natriuretic peptide, human pro B-type natriuretic peptide and human B-type natriuretic peptide in a test sample.

Claims

1. Immunodiagnostic reagents comprising:

(a) a first capture antibody specific for human NT-pro B-type natriuretic peptide (“

human NT-proBNP”

);

(b) a second capture antibody which is specific to each of human pro B-type natriuretic peptide (“

human proBNP”

) and human brain natriuretic peptide (“

hBNP”

);

(c) a first detection antibody which is specific to each of human NT-proBNP and human proBNP; and

(d) a second detection antibody which is specific to hBNP.

2. The immunodiagnostic reagents of claim 1, wherein the first capture antibody is antibody 15F11.

3. The immunodiagnostic reagents of claim 1, wherein the second capture antibody is antibody 8.1.

4. The immunodiagnostic reagents of claim 1, wherein the first detection antibody is antibody 15C4.

5. The immunodiagnostic reagents of claim 1, wherein the second detection antibody is antibody 201.3.

6. A kit for detecting the amount of human NT-pro B-type natriuretic peptide (“

human NT-proBNP”

), human pro B-type natriuretic peptide (“

human proBNP”

) and human brain natriuretic peptide (“

hBNP”

) in a test sample, said kit comprising;

(a) instructions for conducting the assay of the test sample; and

(b) immunodiagnostic reagents comprising;

(i) a first capture antibody specific for human NT-proBNP;

(ii) a second capture antibody which is specific to each of human proBNP and hBNP;

(iii) a first detection antibody which is specific to each of human NT-proBNP and human proBNP; and

(iv) a second detection antibody which is specific to hBNP.

7. The kit of claim 6, wherein the first capture antibody is antibody 15F11.

8. The kit of claim 6, wherein the second capture antibody is antibody 8.1.

9. The kit of claim 6, wherein the first detection antibody is antibody 15C4.

10. The kit of claim 6, wherein the second detection antibody is antibody 201.3.