Method for Sequencing a Polynucelotide Template
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Abstract
The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
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Citations
58 Claims
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1-26. -26. (canceled)
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27. A method for paired-end sequencing of a target polynucleotide comprising the steps of:
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(a) providing a target polynucleotide attached to a support comprising first and second template strands, wherein the first and second template strands comprise first and second regions, respectively, to be sequenced; (b) performing a first sequencing reaction, wherein nucleotides are sequentially added to a first sequencing primer hybridized to the first template strand to determine the sequence of the first region; (c) separating the first region from the first sequencing primer after the sequencing reaction of step (b); and (d) performing a second sequencing reaction, wherein nucleotides are sequentially added to a second sequencing primer hybridized to the second template strand to determine the sequence of the second region. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 55, 56, 57, 58)
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Specification