METHOD OF MANUFACTURING A SCAFFOLD FOR TISSUE ENGINEERING OR REPAIR
First Claim
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1. A method of manufacturing a scaffold for tissue engineering or repair, comprising:
- providing a mixture comprising at least one degradable hydrogel, at least one kind of degradable particle and one or more species of living cells to form a hydrogel comprising the at least one kind of degradable particle and the one or more species of living cells, wherein the degradable particle comprises a material which degrades faster than the degradable hydrogel;
incubating the mixture under conditions which allow proliferation of the one or more species of living cells and degradation of the at least one kind of degradable particle in the hydrogel to allow the at least one kind of degradable particle to degrade and to allow the one or more species of living cells to proliferate;
degrading at least partially the at least one degradable hydrogel of the incubated mixture; and
adding one or more species of living cells after degrading at least partially the at least one degradable hydrogel of the incubated mixture, wherein the one or more species of living cells added after degrading at least partially the at least one degradable hydrogel of the incubated mixture comprises the same or different species of cells than the cells already mixed into the mixture together with the at least one degradable hydrogel and the at least one kind of degradable particle to obtain the scaffold.
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Abstract
The invention relates to a method of manufacturing a scaffold for tissue engineering or repair. The method includes providing a mixture comprising a degradable hydrogel, a degradable particle, and living cells. The mixture is then incubated under conditions which allow proliferation of the living cells and degradation of the degradable particle in the hydrogel. The degradable hydrogel of the incubated mixture is then degraded at least partially, followed by adding further living cells to obtain the scaffold.
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15 Claims
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1. A method of manufacturing a scaffold for tissue engineering or repair, comprising:
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providing a mixture comprising at least one degradable hydrogel, at least one kind of degradable particle and one or more species of living cells to form a hydrogel comprising the at least one kind of degradable particle and the one or more species of living cells, wherein the degradable particle comprises a material which degrades faster than the degradable hydrogel; incubating the mixture under conditions which allow proliferation of the one or more species of living cells and degradation of the at least one kind of degradable particle in the hydrogel to allow the at least one kind of degradable particle to degrade and to allow the one or more species of living cells to proliferate; degrading at least partially the at least one degradable hydrogel of the incubated mixture; and adding one or more species of living cells after degrading at least partially the at least one degradable hydrogel of the incubated mixture, wherein the one or more species of living cells added after degrading at least partially the at least one degradable hydrogel of the incubated mixture comprises the same or different species of cells than the cells already mixed into the mixture together with the at least one degradable hydrogel and the at least one kind of degradable particle to obtain the scaffold. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification