FLUORESCENCE ENHANCING PLASMONIC NANOSCOPIC GOLD FILMS AND ASSAYS BASED THEREON
First Claim
1. A microarray comprising:
- (a) a substrate;
(b) a discontinuous gold film applied to said substrate, said discontinuous gold film having isolated island areas of between about 100 nm2 and 250,000 nm2 in area and configured to enhance plasmonic near-infrared fluorescence; and
(c) an array of biological molecules, for use as capture agents specifically binding to an analyte, disposed in discrete locations on the discontinuous gold film and coupled to the discontinuous gold film, whereby near-infrared fluorescence emission caused by an analyte captured by a capture agent is enhanced by the discontinuous gold film.
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Accused Products
Abstract
Disclosed are nanostructured gold films which may be produced by solution-phase depositions of gold ions onto a variety of surfaces. The resulting plasmonic gold films are used for enhanced spectroscopic-based immunoassays in multiplexed microarray format with detection mechanisms based on either surface-enhanced Raman scattering or near-infrared fluorescence enhancement. The preparation of the films and subsequent modifications of the gold film surfaces afford increased sensitivity for various microarrays. The films are discontinuous, forming gold “islands.” Sensitivity, size, shape, and density of the nanoscopic gold islands comprising the discontinuous nanostructured gold film are controlled to enhance the intensity of Raman scattering and fluorescence in the near-infrared, allowing for improved measurements in clinical diagnostic or biomedical research applications.
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Citations
35 Claims
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1. A microarray comprising:
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(a) a substrate; (b) a discontinuous gold film applied to said substrate, said discontinuous gold film having isolated island areas of between about 100 nm2 and 250,000 nm2 in area and configured to enhance plasmonic near-infrared fluorescence; and (c) an array of biological molecules, for use as capture agents specifically binding to an analyte, disposed in discrete locations on the discontinuous gold film and coupled to the discontinuous gold film, whereby near-infrared fluorescence emission caused by an analyte captured by a capture agent is enhanced by the discontinuous gold film. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for preparing a microarray, comprising:
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(a) applying to a substrate a solution containing gold ions; (b) precipitating the gold ions from solution onto the substrate using a basic solution; (c) reducing the gold ions precipitated onto the substrate in step (b) to produce on the substrate Au(0) seed particles; (d) adding gold ions from solution to the gold seeds from step (c) together with a reducing agent to grow isolated island areas in a discontinuous film; and (e) applying to the discontinuous gold film an array of biological molecules for use as capture agents specifically binding to an analyte, disposed as different molecular species in discrete locations on the discontinuous gold film and coupled to the discontinuous gold film, whereby near-infrared fluorescence emission caused by an analyte captured by a capture agent is enhanced by the discontinuous gold film. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A method of detecting one or more analytes in a sample, comprising:
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(a) providing a microarray having (i.) a substrate; (ii.) a discontinuous gold film applied to said substrate, said gold film having isolated island areas of between about 100 nm2 and 250,000 nm2 in area, configured to enhance plasmonic near-infrared fluorescence; and (iii.) an array of biological molecules, for use as capture agents specifically binding to an analyte, disposed as different molecular species in discrete locations on the discontinuous gold film and coupled to the discontinuous gold film, whereby near-infrared fluorescence emission caused by an analyte captured by a capture agent is enhanced by the discontinuous gold film; (b) applying to said microarray said sample and a near-infrared fluorphore label for said one or more analytes; and (c) detecting fluorescence emission from fluorophore label bound to one or more analytes. - View Dependent Claims (29, 30, 31, 32, 33, 34, 35)
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Specification