GEL ELECTROPHORESIS, IMAGINE, AND ANALYSIS METHODS, DEVICES, SYSTEMS, AND MATERIALS

• US 20130175172A1
• Filed: 12/17/2012
• Published: 07/11/2013
• Est. Priority Date: 08/24/2009
• Status: Active Grant

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: UPDYKE, Timothy, Predki, Paul, Gonzalez, Evangeline, Simons, Tad, Lowe, Randall
• Granted Patent: US 9,097,663 B2
• Time in Patent Office: Days
• US Class Current: 204/457
• CPC Class Codes: G01N 27/44721 by optical means
• CPC Class Codes: G01N 27/44743 Introducing samples
• CPC Class Codes: G01N 27/44778 on a common gel carrier, i.e. 2D gel electrophoresis

Abstract

The present teachings provide methods, devices, systems, and materials for performing electrophoresis in an automated fashion. The electrophoresis system may simultaneously image gel during an electrophoresis run. In some embodiments, the electrophoresis system may analyze an imaged gel during or after electrophoresis. The device may comprise a gel processing system, a gel illumination system, an image capture system, and an image analysis all housed within a housing.

Claims

1. A gel electrophoresis device comprising:

a housing;

a gel processing system;

a gel illumination system;

an image capture system; and

an image analysis system;

wherein the gel processing system, the gel illumination system, the image capture system, and the image analysis system are all housed within the housing.

2. The gel electrophoresis device of claim, 1, wherein the systems are operably associated and automated.

3. The device of claim 1, wherein the gel processing system comprises at least two gel buffer reservoirs, at least two gel holders, and at least one anode and at least one cathode for each of the at least two gel holders.

4. The device of claim 1, wherein the gel illumination system comprises at least one light source configured to illuminate at least one electrophoresis gel when the gel is placed in the gel processing system.

5. The device of claim 4, wherein the at least one light source comprises at least one blue light source.

6. The device of claim 5, wherein the at least one blue light source emits blue light in a wavelength range of from about 440 nm to about 490 nm.

7. The device of claim 4, wherein the at least one blue light source comprises at least one LED blue light source.

8. The device of claim 4, wherein the at least one light source comprises at least two light sources.

9. The device of claim 4, further comprising at least one light intensity controlling component.

10. The device of claim 4, wherein the at least one light source is pre-configured such that no additional manipulation of the gel illumination system is required, beyond automatic or manual activation of the system, to provide sufficient illumination for successful image capture at the position at which an electrophoresis gel is placed into the device when the device is being used for electrophoresis.

11. The device of claim 1, wherein the image capture system comprises at least one digital camera.

12. The device of claim 11, wherein the at least one digital camera is pre-configured such that no additional manipulation of the image capture system is required, beyond automatic or manual activation of the system, to provide successful image capture at the position at which an electrophoresis gel is placed into the device when the device is being used for electrophoresis.

13. The device of claim 11, wherein the image capture system is configured to provide real time imaging of at least one electrophoresis gel during electrophoresis.

14. A method of performing gel electrophoresis comprising:

performing electrophoresis on at least one labeled sample in a gel electrophoresis device comprising a gel processing system and an image capture system; and

imaging the at least one labeled sample with the image capture system;

wherein at least the performing and imaging steps are operably associated with each other and automated.

15. The method of claim 14, further comprising forming an analyzed image by analyzing the imaged sample with an image analysis system operably associated with the image capture system.

16. The method of claim 15, further comprising printing a hard copy of the analyzed image.

17. The method of claim 14, further comprising electrophoresing a labeled protein standard simultaneously with the labeled sample.

18. The method of claim 14, further comprising determining the absolute amount of at least one labeled protein in the sample.

19. The method of claim 14, wherein the method is performed in less than about 30 minutes.

20. The method of claim 14, wherein the method is performed in less than about 15 minutes.

21. The method of claim 14, wherein the imaging comprises exposing the labeled sample to at least one blue light source LED blue light source.

22. The method of claim 21, wherein the at least one LED blue light source emits blue light in a wavelength range of from about 440 nm to about 490 nm.

23. The method of claim 21, wherein the image capture system captures images in real-time during the electrophoresis on the at least one labeled sample.