ULTRASONIC CAVITATION DERIVED STROMAL OR MESENCHYMAL VASCULAR EXTRACTS AND CELLS DERIVED THEREFROM OBTAINED FROM ADIPOSE TISSUE AND USE THEREOF

• US 20130189234A1
• Filed: 01/18/2013
• Published: 07/25/2013
• Est. Priority Date: 12/27/2010
• Status: Abandoned Application

• Current Assignee: Stroma Cell Therapeutics LLC
• Original Assignee: Intellicell Biosciences, Inc.
• Inventors: Victor, Steven
• Application Number:
  US13/745,367
• Publication Number:
  US 20130189234A1
• Time in Patent Office: Days
• US Class Current: 424/93.7
• CPC Class Codes: A61K 35/35 Fat tissue; Adipocytes; Stromal cells; Connective tissues adipose-derived stem cells A61K35/28; collagen A61K38/39
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 25/06 Antimigraine agents
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: C12N 5/0667 Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
• CPC Class Codes: G01N 2333/70503 Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
• CPC Class Codes: G01N 2333/70596 Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025

Abstract

Methods of treating using adipose tissue using ultrasonic cavitation to dissociate the fat cells and blood vessels contained within adipose tissue and thereby obtain mesenchymal or stromal vascular fractions for use in human subjects are provided. These methods preferably do not include the use of any exogenous dissociating enzymes such as collagenase and result in increased numbers of the cells which constitute the mesenchymal or stromal vascular fractions (about 10-fold greater) than methods which use collagenase to isolate these cells.

Claims

1. A method of recovering a stromal or mesenchymal vascular fraction from blood vessels contained in or proximate to adipose tissue which method comprises treating an adipose tissue containing sample with ultrasonic cavitation under conditions whereby that the fat cells and blood vessels in the sample are exploded or lysed thereby dissociating or releasing substantial numbers of intact stromal vascular or mesenchymal vascular cells from the lysed blood vessels contained in the ultrasonicated adipose tissue while substantially maintaining the viability of the cells constituting the stromal or mesenchymal fraction.

27. A stromal vascular fraction derived from adipose tissue prepared by the method according to claim 1, wherein said stromal vascular fraction does not comprise any exogenous collagenase.

28. The stromal vascular fraction according to claim 27, which contains stem or other cells that express at least one protein selected from the group consisting of CD13, CD29, CD34, CD36, CD44, CD49d, CD54, CD58, CD71, CD73, Thy-1 or CD90, CD105, CD106, CD151 and SH3.

29. The stromal vascular fraction according to claim 27, which contains stem or other cells that express at least one of CD13, CD29, CD34, CD36, CD44, CD49d, CD54, CD58, CD71, CD73, CD90, Thy-1 or CD105, CD106, CD151 and SH3 or any combination thereof.

30. The stromal vascular fraction according to claim 27, which contains stem cells that express at least one protein selected from the group consisting of CD31, CD45, CD117 and CD146.

31. The stromal vascular fraction according to claim 27, which contains stem cells that do not express CD56.

32. The stromal vascular fraction according to claim 27, which contains stem cells which does not express at least one protein selected from the group consisting of CD3, CD4, CD14, CD15, CD16, CD19, CD33, CD38, CD56, CD61, CD62e, CD62p, CD69, CD104, CD135 and CD144.

33. The stromal vascular fraction according to claim 27, which contains stem cells that do not express CD3, CD4, CD14, CD15, CD16, CD19, CD33, CD38, CD56, CD61, CD62e, CD62p, CD69, CD104, CD135 and CD144.

34. The stromal vascular fraction according to claim 27, which contains stem cells which express CD49d and do not express CD56.

50. A cosmetic procedure or therapy to treat a human disease or medical condition comprising administering the stromal vascular fraction of claim 27.

51. The method of claim 50, wherein said methods of use include treatment alone or in combination with tissue fillers, use for treating gum recession, treating loss of bone, including the jaw, treatment of orthopedic problems, treatment of arthritis, treatment of migraine, treatment of multiple sclerosis, treatment of autism, treatment of diabetes, treatment of wounds, treatment of ulcers, treatment of COPD, treatment of plantar fascitis, treatment of rotator cuff, and treatment of tennis elbow.

2-26. -26. (canceled)

35-49. -49. (canceled)