Optical Assay Device with Pneumatic Sample Actuation
First Claim
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1. A testing device comprising:
- an entry port configured to receive a test sample into a holding chamber;
a first conduit comprising at least one lateral flow test strip; and
a displacement device configured to move a portion of said test sample from said holding chamber into said first conduit.
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Abstract
This invention relates generally to devices and methods for performing optical and electrochemical assays and, more particularly, to test devices, e.g., cartridges, methods and systems, wherein the test devices have an entry port configured to receive a test sample into a holding chamber; a first conduit having at least one lateral flow test strip; and a displacement device, such as a pneumatic pump, configured to move a portion of said test sample from said holding chamber into said first conduit. The present invention is particularly useful for performing immunoassays and/or electrochemical assays at the point-of-care.
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Citations
38 Claims
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1. A testing device comprising:
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an entry port configured to receive a test sample into a holding chamber; a first conduit comprising at least one lateral flow test strip; and a displacement device configured to move a portion of said test sample from said holding chamber into said first conduit. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A testing system comprising:
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a testing device comprising; an entry port configured to receive a test sample into a holding chamber; a first conduit comprising at least one lateral flow test strip; and displacement device configured to move a portion of said test sample from said holding chamber into said first conduit; and an instrument comprising a receiving port configured to receive and engage said testing device. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A computer system for testing an analyte, the system comprising:
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a CPU, a computer readable memory and a computer readable storage media; first program instructions to actuate a pneumatic pump using a processor to move a portion of a test sample in a testing device from a holding chamber into a first conduit, said first conduit comprising a detection zone including a plurality of capture antibodies; and second program instructions to capture an image of said detection zone, wherein said first and said second program instructions are stored on the computer readable storage media for execution by the CPU via the computer readable memory. - View Dependent Claims (28, 29)
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30. An analyte test cartridge comprising:
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a sample entry port configured to receive a test sample; a sample holding chamber; a labeled antibody for an analyte in said test sample; a displacement device configured to move said test sample through at least a portion of said test cartridge; a conduit; and a porous plug with a capture antibody for said analyte, wherein said sample holding chamber is connected to said closable sample entry port, said displacement device, and said conduit;
wherein said labeled antibody is coated on at least a portion of said conduit;
wherein said labeled antibody is configured to dissolve into said test sample; and
wherein said porous plug with said capture antibody is positioned in said conduit. - View Dependent Claims (31, 32, 33, 34, 35)
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36. A cartridge for detection of an analyte in a sample, the cartridge comprising:
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an entry port for receiving said sample into a holding chamber; a conduit with a bead reagent that is configured to dissolve into said sample; a wicking matrix comprising an immobilized antibody that is configured to bind with said analyte; and a displacement device configured to move said sample into said conduit, mix said bead reagent with said sample to form a complex of said analyte with said bead reagent, and move said sample to said wicking matrix to capture said complex.
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37. A method of performing an analyte assay comprising:
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introducing a sample into a sample holding chamber of a cartridge through a sample entry port; activating a pump to move at least a portion of said sample into a conduit;
oscillating said sample to dissolve an optically labeled antibody into said sample and to permit binding of said optically labeled antibody with an analyte to form a complex;waiting a predetermined amount of time; activating said pump after waiting said predetermined amount of time to move said sample to a porous plug in said conduit, wherein at least a portion of said porous plug comprises an immobilized antibody configured to bind to said complex; and activating an optical detector aligned with said porous plug to determine a presence or amount of retained optically labeled antibody.
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38. A computer program product comprising a computer usable storage medium having readable program code embodied in the storage medium, wherein the computer program product includes at least one component operable to:
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actuate a pneumatic pump to move a portion of a test sample in a testing device from a holding chamber into a conduit, a portion of said conduit comprising a capture antibody; and actuate an imager aligned with said portion of said conduit to capture an image of said portion of said conduit.
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Specification