MANUFACTURING METHOD FOR SUGAR SOLUTION AND DEVICE FOR SAME

• US 20130203117A1
• Filed: 03/14/2011
• Published: 08/08/2013
• Est. Priority Date: 03/15/2010
• Status: Active Grant

• Current Assignee: Toray Industries Incorporated
• Original Assignee: Toray Industries Incorporated
• Inventors: Kurihara, Hiroyuki, Minamino, Atsushi, Yamamoto, Yuki, Yamada, Katsushige
• Granted Patent: US 9,624,516 B2
• Time in Patent Office: Days
• US Class Current: 435/72
• CPC Class Codes: C12M 21/18 Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
• CPC Class Codes: C12M 29/04 Filters; Permeable or porous membranes or plates, e.g. dialysis
• CPC Class Codes: C12M 33/14 with filters, sieves or membranes
• CPC Class Codes: C12M 47/10 Separation or concentration of fermentation products bioreactors combined with means for distillation or extraction of liquid fuel C12M43/02
• CPC Class Codes: C12P 19/00 Preparation of compounds containing saccharide radicals ketoaldonic acids C12P7/58
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C13K 1/02 obtained by saccharification of cellulosic materials manufacture of fodder A23K10/32
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

A method produces a sugar liquid by repeating a sugar liquid production process including (1) to (3): (1) adding a filamentous fungus-derived cellulase to cellulose to perform primary hydrolysis; (2) adding a fresh filamentous fungus-derived cellulase to the hydrolysate in Step (1) to perform secondary hydrolysis; and (3) subjecting the hydrolysate in Step (2) to solid-liquid separation to obtain a sugar liquid, from which a recovered enzyme is obtained; wherein the recovered enzyme obtained in Step (3) is used for Step (1) of the next and later sugar liquid production processes.

Claims

1. A method for producing a sugar liquid by repeating a sugar liquid production process comprising Steps (1) to (3) below:

(1) adding a filamentous fungus-derived cellulase to cellulose to perform primary hydrolysis;

(2) adding a fresh filamentous fungus-derived cellulase to the hydrolysate in Step (1) to perform secondary hydrolysis; and

(3) subjecting the hydrolysate in Step (2) to solid-liquid separation to obtain a sugar liquid, from which a recovered enzyme is obtained;

wherein said recovered enzyme obtained in Step (3) is used for Step (1) of the-next and later sugar liquid production processes.

2. The method according to claim 1, wherein, as said filamentous fungus-derived cellulase in the Step (1) of the sugar liquid production process, an enzyme component recovered from a cellulose hydrolysate produced by a filamentous fungus-derived cellulase is used.

3. The method according to claim 1, wherein said filamentous fungus-derived cellulase in the Step (1) or (2) comprises a component derived from a culture liquid of a microorganism belonging to the genus Trichoderma.

4. The method according to claim 1, wherein said recovered enzyme comprises xylanase and/or xylosidase.

5. The method according to claim 1, wherein said recovered enzyme comprises a water-insoluble filamentous fungus-derived cellulase.

6. The method according to claim 1, wherein said cellulose is a processed product obtained by subjecting a cellulose-containing biomass to alkaline treatment, hydrothermal treatment or dilute sulfuric acid treatment.

7. The method according to claim 1, wherein amounts of enzyme added in said primary hydrolysis and said secondary hydrolysis satisfy a following relation:

an amount of said recovered enzyme added in Step (1)>

an amount of said fresh enzyme added in Step (2).

8. The method according to claim 1, wherein recovery of said filamentous fungus-derived cellulase in the Step (3) is carried out by filtering said sugar liquid through an ultrafiltration membrane and recovering said cellulase from the feed side.

13. The method according to claim 2, wherein said filamentous fungus-derived cellulase in the Step (1) or (2) comprises a component derived from a culture liquid of a microorganism belonging to the genus Trichoderma.

14. The method according to claim 2, wherein said recovered enzyme comprises xylanase and/or xylosidase.

15. The method according to claim 3, wherein said recovered enzyme comprises xylanase and/or xylosidase.

16. The method according to claim 2, wherein said recovered enzyme comprises a water-insoluble filamentous fungus-derived cellulase.

17. The method according to claim 3, wherein said recovered enzyme comprises a water-insoluble filamentous fungus-derived cellulase.

18. The method according to claim 4, wherein said recovered enzyme comprises a water-insoluble filamentous fungus-derived cellulase.

9. An apparatus for producing a sugar liquid by a method comprising hydrolyzing cellulose, comprising:

a hydrolysis tank to which a recovered enzyme feed pipe and a fresh enzyme feed pipe are connected;

a device for solid-liquid separation of a hydrolysate;

a sugar liquid-retaining tank having a water supply pipe that washes an ultrafiltration membrane and/or for removing recovered enzyme retained in a circulation pipe; and

an ultrafiltration membrane device for separation of enzyme and a sugar liquid.

11. The apparatus according to claim 9, further comprising a reverse osmosis membrane and/or nanofiltration membrane device(s) that concentrate said sugar liquid.

10. An apparatus for producing a sugar liquid by a method comprising hydrolyzing cellulose, comprising:

a cellulose/recovered enzyme-mixing device that mixes recovered enzyme and cellulose to perform primary hydrolysis;

a hydrolysis tank to which a cellulose/recovered enzyme mixture supply pipe and a fresh enzyme feed pipe are connected;

a device for solid-liquid separation of a hydrolysate;

a sugar liquid-retaining tank having a water supply pipe for washing an ultrafiltration membrane and/or removing recovered enzyme retained in a circulation pipe; and

an ultrafiltration membrane device for separation of enzyme and a sugar liquid.

12. The apparatus according to claim 10, further comprising a reverse osmosis membrane and/or nanofiltration membrane device(s) that concentrate said sugar liquid.