MICROFLUIDIC CARTRIDGE FOR PROCESSING AND DETECTING NUCLEIC ACIDS
First Claim
1. A cartridge, configured to facilitate processing and detecting nucleic acid in a sample, comprising:
- a first layer comprising a sample port and a fluid port;
an intermediate substrate coupled to the first layer and defining a waste chamber; and
a fluidic pathway, formed by at least a portion of the first layer, wherein the fluidic pathway is fluidically coupled to the sample port and the fluid port, and is configured to transfer waste fluid to the waste chamber.
4 Assignments
0 Petitions
Accused Products
Abstract
A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.
48 Citations
29 Claims
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1. A cartridge, configured to facilitate processing and detecting nucleic acid in a sample, comprising:
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a first layer comprising a sample port and a fluid port; an intermediate substrate coupled to the first layer and defining a waste chamber; and a fluidic pathway, formed by at least a portion of the first layer, wherein the fluidic pathway is fluidically coupled to the sample port and the fluid port, and is configured to transfer waste fluid to the waste chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A cartridge, configured to facilitate processing and detecting of nucleic acids, comprising:
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a first layer and an intermediate substrate, coupled to the first layer, wherein the intermediate substrate defines a waste chamber; a first fluidic pathway, formed by at least a portion of the first layer; and a second fluidic pathway, formed by at least a portion of the first layer, wherein the first fluidic pathway is configured to transfer waste to the waste chamber and wherein the second fluidic pathway is configured to transfer waste to the waste chamber. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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Specification