MOLECULAR DIAGNOSTIC SCREENING ASSAY
First Claim
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1. A method for disease screening, the method comprising:
- obtaining nucleic acid from a sample;
incubating the nucleic acid with first and second sets of binders, wherein members of the first set bind to different regions of a target nucleic acid, members of the second set bind to different regions of a reference nucleic acid, and members of the first set comprise a detectable label that is different than members of the second set;
removing unbound binders;
detecting the labels; and
identifying a positive screen based upon differential presence of label between said first and second sets.
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Abstract
The invention generally relates to method for screening for a condition in a subject. In certain embodiments, methods of the invention involve obtaining a pool of nucleic acids from a sample, incubating the nucleic acids with first and second sets of binders, in which the first set binds uniquely to different regions of a target nucleic acid in the pool, the second set binds uniquely to different regions of a reference nucleic acid in the pool, and the first and second sets include different detectable labels, removing unbound binders, detecting the labels, and screening for a condition based upon results of the detecting step.
71 Citations
50 Claims
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1. A method for disease screening, the method comprising:
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obtaining nucleic acid from a sample; incubating the nucleic acid with first and second sets of binders, wherein members of the first set bind to different regions of a target nucleic acid, members of the second set bind to different regions of a reference nucleic acid, and members of the first set comprise a detectable label that is different than members of the second set; removing unbound binders; detecting the labels; and identifying a positive screen based upon differential presence of label between said first and second sets. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 49, 50)
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41. A method for screening for a condition in a subject, the method comprising:
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obtaining a pool of different nucleic acid from a sample; compartmentalizing the pool of nucleic acids into compartmentalized portions, the compartmentalized portions comprising, on average, either a first set of binders or a second set of binders, wherein the first and second sets comprise different detectable labels and the first and second sets bind to different nucleic acids in the pool; amplifying only binders in the compartmentalized portions that bound to the nucleic acid; detecting the labels; and screening for a condition based upon results of the detecting step. - View Dependent Claims (42, 43, 44, 45, 46, 47, 48)
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Specification