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Direct Cloning

  • US 20130210681A1
  • Filed: 06/10/2011
  • Published: 08/15/2013
  • Est. Priority Date: 06/10/2010
  • Status: Active Grant
First Claim
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1. A method for performing homologous recombination between at least a first nucleic acid molecule and a second nucleic acid molecule which share at least one region of sequence homology, wherein the method comprises bringing the first nucleic acid molecule into contact with the second nucleic acid molecule in the presence of a 5′

  • to 3′

    exonuclease and an annealing protein;

    wherein the 5′

    to 3′

    exonuclease comprises a region having 5′

    to 3′

    exonuclease activity and at least;

    i) amino acids 564-587 of SEQ ID NO;

    1;

    orii) a 24 amino acid sequence having at least 70% identity to amino acids 564-587 of SEQ ID NO;

    1 over the entire length of the 24 amino acid sequence.

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