IN VITRO EVOLUTION IN MICROFLUIDIC SYSTEMS
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Abstract
The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
42 Citations
120 Claims
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1-107. -107. (canceled)
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108. A method of producing a library of entities, comprising the steps of:
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producing a plurality of microcapsules, each microcapsule comprising at least one entity of a species; and pooling the microcapsules into one or more common compartments, thereby providing a library of encapsulated entities, wherein at least one step of the method is performed under microfluidic control. - View Dependent Claims (109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120)
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Specification