POLYMERIC VDAT NANOPARTICLES FOR USE IN BIOSENSORS

• US 20130220835A1
• Filed: 02/29/2012
• Published: 08/29/2013
• Est. Priority Date: 02/29/2012
• Status: Abandoned Application

• Current Assignee: LifeScan Scotland Limited (Johnson & Johnson)
• Original Assignee: LifeScan Scotland Limited (Johnson & Johnson)
• Inventors: LIU, Zuifang, SIMPSON, Louise, CARDOSI, Marco
• Application Number:
  US13/408,970
• Publication Number:
  US 20130220835A1
• Time in Patent Office: Days
• US Class Current: 205/782
• CPC Class Codes: C12Q 1/001 Enzyme electrodes

Abstract

A biosensor (such as an electrochemical-based analytical test strip configured for the determination of glucose in a whole blood sample) includes a substrate, an electrode disposed on the substrate and a uric acid scavenger layer containing polymeric vinyl-4,6-diamino-1,3,5-triazine (polyVDAT) nanoparticles. Aqueous compositions useful in, for example, the manufacturing of such biosensors include polyVDAT nanoparticles and water with the polyVDAT nanoparticles being present as a dispersion in the water. A method for determining an analyte in a bodily fluid sample containing uric acid includes applying a bodily fluid sample containing uric acid to a biosensor such that the bodily fluid sample comes into contact with a uric acid scavenger layer containing polymeric vinyl-4,6-diamino-1,3,5-triazine (polyVDAT) nanoparticles and determining the analyte based on an electronic signal produced by the biosensor.

Claims

1. A biosensor comprising:

a substrate;

at least one electrode disposed on the substrate; and

a uric acid scavenger layer containing polymeric nanoparticles including polymerized vinyl-4,6-diamino-1,3,5-triazine (polyVDAT nanoparticles) disposed above the at least one electrode.

2. The biosensor of claim 1 wherein the polyVDAT nanoparticles have a diameter in the range of 30 nanometers to 1000 nanometers.

3. The biosensor of claim 1 wherein the polyVDAT nanoparticles are essentially spherical in shape.

4. The biosensor of claim 1 wherein the polyVDAT nanoparticles include only polymerizedvinyl-4,6-diamino-1,3,5-triazine.

5. The biosensor of claim 4 wherein the polyVDAT nanoparticles are of the following chemical structure:

6. The biosensor strip of claim 1 wherein the polyVDAT nanoparticles are a copolymer of vinyl-4,6-diamino-1,3,5-triazine and one or more monomers.

7. The biosensor of claim 6 wherein the monomer is at least one of styrene and methyl methacrylate.

8. The biosensor of claim 1 wherein the polyVDAT nanoparticles are synthesized from a mixture of vinyl-4,6-diamino-1,3,5-triazine and a crosslinking compound.

9. The biosensor of claim 1 wherein the at least one electrode includes a working electrode and a counter/reference electrode and the uric acid scavenger layer is disposed above at least the working electrode.

10. The biosensor of claim 9 wherein the biosensor is configured as an electrochemical-based analytical test strip and includes an enzymatic reagent layer.

11. The biosensor of claim 10 wherein the uric acid scavenger layer containing polyVDAT nanoparticles is disposed above the enzymatic reagent layer and the working electrode.

12. The biosensor of claim 10 wherein the uric acid scavenger layer containing polyVDAT nanoparticles is disposed between the enzymatic reagent layer and the working electrode.

13. The biosensor of claim 10 wherein the enzymatic reagent layer that is integrated with the scavenger layer containing polyVDAT nanoparticles.

14. The biosensor of claim 10 wherein the electrochemical-based analytical test strip is configured for the determination of glucose in a whole blood sample.

15. The biosensor of claim 1 wherein the polyVDAT nanoparticles are irregular in shape.

16. An aqueous vinyl-4,6-diamino-1,3,5-triazine (VDAT) composition for use in analytical test strips, the aqueous vinyl-4,6-diamino-1,3,5-triazine (VDAT) composition comprising:

polymeric nanoparticles including polymerized vinyl-4,6-diamino-1,3,5-triazine (polyVDAT nanoparticles); and

water,

wherein the polyVDAT nanoparticles are present as a dispersion in the water.

17. The aqueous vinyl-4,6-diamino-1,3,5-triazine (VDAT) composition of claim 16 wherein the polyVDAT nanoparticles have a diameter of less than 1000 nanometers.

18. The aqueous vinyl-4,6-diamino-1,3,5-triazine (VDAT) composition of claim 16 wherein the polyVDAT nanoparticles have a diameter in the range of 30 nanometers to 1000 nanometers.

19. The aqueous vinyl-4,6-diamino-1,3,5-triazine (VDAT) composition of claim 16 wherein the polyVDAT nanoparticles are essentially spherical in shape.

20. The aqueous vinyl-4,6-diamino-1,3,5-triazine (VDAT) composition of claim 16 wherein polyVDAT nanoparticles are irregular in shape.

21. The aqueous vinyl-4,6-diamino-1,3,5-triazine (VDAT) composition of claim 16 further including a binder.

22. The aqueous vinyl-4,6-diamino-1,3,5-triazine (VDAT) composition of claim 16 wherein the aqueous vinyl-4,6-diamino-1,3,5-triazine (VDAT) composition has a pH between 4.0 and 14.0.

23. The aqueous vinyl-4,6-diamino-1,3,5-triazine (VDAT) composition of claim 16 wherein the polyVDAT nanoparticles include only polymerized vinyl-4,6-diamino-1,3,5-triazine.

24. The aqueous vinyl-4,6-diamino-1,3,5-triazine (VDAT) composition of claim 16 wherein the polyVDAT are a copolymer of vinyl-4,6-diamino-1,3,5-triazine and one or more monomers.

25. The aqueous vinyl-4,6-diamino-1,3,5-triazine (VDAT) composition of claim 16 wherein the polyVDAT nanoparticles are synthesized from a mixture of vinyl-4,6-diamino-1,3,5-triazine and a crosslinking compound.

26. A method for determining an analyte in a bodily fluid sample containing uric acid, the method comprising:

applying a bodily fluid sample containing uric acid to a biosensor such that the bodily fluid sample comes into contact with a uric acid scavenger layer containing polymeric nanoparticles that include polymerized vinyl-4,6-diamino-1,3,5-triazine (polyVDAT nanoparticles); and

determining the analyte based on an electronic signal produced by the biosensor.

27. The method of claim 26 wherein the polyVDAT nanoparticles have a diameter of less than 1000 nanometers.

28. The method of claim 26 wherein the polyVDAT nanoparticles have a diameter in the range of 30 nanometers to 1000 nanometers.

29. The method of claim 26 wherein the polyVDAT nanoparticles are essentially spherical in shape.

30. The method of claim 26 wherein the biosensor is configured as an electrochemical-based analytical test strip.

31. The method of claim 30 wherein the bodily fluid sample is a whole blood sample and the analyte is glucose.

32. The method of claim 26 wherein the polyVDAT nanoparticles include only polymerized vinyl-4,6-diamino-1,3,5-triazine.

33. The method of claim 26 wherein the polyVDAT nanoparticles are a copolymer of vinyl-4,6-diamino-1,3,5-triazine and one or more monomers.

34. The method of claim 26 wherein the polyVDAT nanoparticles are synthesized from a mixture of vinyl-4,6-diamino-1,3,5-triazine and a crosslinking compound.

35. The method of claim 26 wherein the polyVDAT nanoparticles are of the following chemical structure:

36. The method of claim 26 wherein the polyVDAT nanoparticles are irregular in shape.