NUCLEIC ACID AMPLIFICATION
First Claim
1. A method for nucleic acid amplification, comprising:
- (a) distributing at least two polynucleotides into an array of reaction chambers by introducing a single one of said polynucleotides into at least two of said reaction chambers; and
(b) forming at least two substantially monoclonal nucleic acid populations by amplifying the polynucleotides within said at least two reaction chambers, wherein the said at least two reaction chambers are in fluid communication with each other during the amplifying.
2 Assignments
0 Petitions
Accused Products
Abstract
In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.
-
Citations
20 Claims
-
1. A method for nucleic acid amplification, comprising:
-
(a) distributing at least two polynucleotides into an array of reaction chambers by introducing a single one of said polynucleotides into at least two of said reaction chambers; and (b) forming at least two substantially monoclonal nucleic acid populations by amplifying the polynucleotides within said at least two reaction chambers, wherein the said at least two reaction chambers are in fluid communication with each other during the amplifying. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19, 20)
-
-
18. The method of claim 18, wherein the output signal is optionally based on a threshold voltage of the FET.
Specification