Total Internal Reflection Photoacoustic Spectroscopy
First Claim
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1. A system for conducting total internal reflection photoacoustic spectroscopy (TIRPAS), the system comprising:
- a pulsed laser source for emitting a thermally or stress confined laser beam;
a prism comprising a surface to be in contact with a sample that is to be subjected to TIRPAS, wherein the laser beam and the prism, when conducting TIRPAS, are configured such that the laser beam travels through the prism to a location at an interface between the prism and the sample at an angle of incidence such that an evanescent field extending into the sample forms; and
a detector for determining whether a photoacoustic response to the evanescent field is generated by an analyte in the sample.
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Abstract
A system and method of conducting total internal reflection photoacoustic spectroscopy (TIRPAS) comprising using a pulsed laser source to emit a thermally or stress confined laser beam at a prism comprising a surface to be in contact with a sample such that the laser beam travels through the prism to a location at an interface between the prism and the sample to form an evanescent field extending into the sample to generate a detectable photoacoustic response to the evanescent field by an analyte in the sample.
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Citations
19 Claims
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1. A system for conducting total internal reflection photoacoustic spectroscopy (TIRPAS), the system comprising:
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a pulsed laser source for emitting a thermally or stress confined laser beam; a prism comprising a surface to be in contact with a sample that is to be subjected to TIRPAS, wherein the laser beam and the prism, when conducting TIRPAS, are configured such that the laser beam travels through the prism to a location at an interface between the prism and the sample at an angle of incidence such that an evanescent field extending into the sample forms; and a detector for determining whether a photoacoustic response to the evanescent field is generated by an analyte in the sample.
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2. The system of claim 1, wherein the beam is thermally confined and the pulsed laser source is a Q-switched Nd:
- YAG laser, the prism is a right-angle prism, and the detector is a transducer.
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3. The system of claim 2, wherein the transducer is a piezoelectric copolymer.
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4. The system of claim 1 further comprising an XYZ translational stage and an electric motor to affect movement of the XYZ translational stage to which the prism is to be mounted when conducting TIRPAS to facilitate adjustment of the location at the interface to which the beam travels, the angle of incidence, or both.
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5. The system of claim 1 further comprising one or more of the following:
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a collimator for enhancing the collimation of the laser beam before the laser beam enters the prism; a spatial filter for filtering the portions of the laser beam; a variable aperture for reducing diffraction in the laser beam; a beam expander for expanding the laser beam; and a beam splitter for enhancing the collimation of the laser beam before the laser beam enters the prism.
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6. The system of claim 1 further comprising an amplifier for amplifying the detected photoacoustic response to the evanescent field generated by the analyte in the sample.
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7. The system of claim 6, wherein the amplifier is selected from the group consisting of a broadband amplifier.
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8. The system of claim 1 further comprising a processor configured to detect the photoacoustic response to the evanescent field generated by the analyte.
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9. The system of claim 8, wherein the processor is further configured to analyze the photoacoustic response to the evanescent field generated by the analyte.
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10. A method of conducting total internal reflection photoacoustic spectroscopy (TIRPAS), the method comprising:
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directing a thermally or stress confined laser beam at a prism comprising a surface in contact with a sample at an angle of incidence, wherein the laser beam and the prism are configured such that the laser beam travels through the prism to a location at an interface between the prism and the sample and forms an evanescent field extending into the sample; and determining whether a photoacoustic response to the evanescent field is generated by an analyte in the sample.
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11. The method of claim 10, wherein the laser beam is thermally confined and the pulse laser source is a Q-switched Nd:
- YAG laser, the prism is a right-angle prism, and the detector is a transducer.
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12. The method of claim 11, wherein the transducer is a piezoelectric copolymer.
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13. The method of claim 10 further comprising adjusting the angle of incidence of the laser beam, the location at the interface to which the laser beam travels, or both when conducting TIRPAS.
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14. The method of claim 13, wherein the adjusting step is performed by an XYZ translational stage to which the prism is mounted and an electric motor to affect movement of the XYZ translational stage.
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15. The method of claim 10 further comprising one or more of the following:
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collimating the laser beam before the laser beam enters the prism; filtering a portion of the laser beam with a spatial filter; reducing diffraction in the laser beam with a variable aperture; and expanding the laser beam by using a beam expander.
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16. The method of claim 10 further comprising amplifying the detected photoacoustic response to the evanescent field generated by the analyte in the sample by using an amplifier.
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17. The method of claim 16, wherein the amplifier is a broadband amplifier.
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18. The method of claim 10, wherein the detecting step is performed by a processor.
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19. The method of claim 18, further comprising analyzing the detected photoacoustic response to the evanescent field generated by the analyte with the processor.
Specification