BIOMARKER NORMALIZATION

• US 20130233061A1
• Filed: 03/15/2013
• Published: 09/12/2013
• Est. Priority Date: 08/06/2002
• Status: Abandoned Application

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Sullivan, Benjamin
• Application Number:
  US13/842,474
• Publication Number:
  US 20130233061A1
• Time in Patent Office: Days
• US Class Current: 73/64.47
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 13/04 Investigating osmotic effects
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 27/07 Construction of measuring vessels; Electrodes therefor
• CPC Class Codes: G01N 27/26 by investigating electrochemical variables; by using electrolysis or electrophoresis
• CPC Class Codes: G01N 2800/162 Conjunctival disorders, e.g. conjunctivitis
• CPC Class Codes: G01N 2800/24 Immunology or allergic disorders SLE G01N2800/104
• CPC Class Codes: G01N 33/487 of liquid biological material
• CPC Class Codes: G16B 40/00 ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
• CPC Class Codes: G16B 40/10 Signal processing, e.g. from mass spectrometry [MS] or from PCR

Abstract

A fluid sample is measured with a tear film measuring system that includes is processing device that receives a sample chip comprising a sample region configured to contain an aliquot volume of sample fluid, the processing device configured to perform analyses of osmolarity and of one or more biomarkers within the sample fluid, wherein the analysis of biomarkers includes normalization of biomarker concentration values.

Claims

1-37. -37. (canceled)

38. A device for determining the presence of an analyte in a fluid sample comprising:

a. a collection chamber comprising a hydrophilic polymer;

b. a fluidic channel connected to the collection chamber;

c. a sensing chamber connected to the fluidic channel,

wherein the sensing chamber contains a material that specifically detects the analyte and wherein the sensing chamber is operably linked to a processor that allows detection of the analyte using electrochemical sensing.

39. The device of claim 38, wherein the processor is an electrode system, and wherein the processing of the fluid comprises applying a voltage to the electrode system to induce an electrochemical reaction between the material that specifically detects the analyte and the analyte in the fluid sample and detecting a current produced by the electrochemical reaction from the contact of the analyte with the material that specifically detects the analyte.

40. The device of claim 38, wherein detection of the analyte comprises an enzyme modified electrochemical reaction.

41. The device of claim 38, wherein the hydrophilic polymer comprises polyether block amides (PEBA), block-copolyether-esters (PEE), polylactic acid (PLA), polyurethanes (PU) including aliphatic and thermoplastic polyurethanes, polyglycolic acid (PGA) and other polyesters (PE), polycaprolactone (PCL), polyethersulfones (PES), polycarbonate (PC), or a combination thereof.

43. The method of claim 38, wherein said analyte is a biomarker.

48. A method of determining the concentration of an analyte in a fluid sample comprising:

a. receiving said fluid into the collection chamber of the device of claim 38;

b. transferring the liquid collected in step (a) to a sensing chamber of said device to determine the presence of said analyte in said fluid

c. correlating the determined presence of the analyte in said liquid with a concentration of said analyte in said liquid.

49. The method of claim 48, wherein receiving said fluid in the collection chamber comprises placing the tip of the collection chamber well near the eye region of a subject.

50. The method of claim 48, wherein the sensing chamber comprises a receptor for the biomarker.

51. The method of claim 48, wherein the analyte is a biomarker.

52. The method of claim 51, wherein the biomarker is glucose.

53. The method of claim 48, wherein the fluid sample is tear fluid.

54. The method of claim 53, wherein volume of the tear fluid is less than 20 μ

l.

55. The method of claim 54, wherein the volume of the tear fluid is between about 1 μ

l to about 5 μ

l.

42. The method of claim 42, wherein the sensing chamber comprises a receptor for the biomarker.

45. The method of claim 42, wherein said fluid sample is tear fluid.

46. The method of claim 45, wherein volume of the tear fluid is less than 20 μ

l.

47. The method of claim 46, wherein the volume of the tear fluid is between about 1 μ

l to about 5 μ

l.

44. The method of claim 44, wherein the biomarker is glucose.