CORRECTION METHOD OF FLUORESCENCE SENSOR AND FLUORESCENCE SENSOR

• US 20130234045A1
• Filed: 04/24/2013
• Published: 09/12/2013
• Est. Priority Date: 10/26/2010
• Status: Abandoned Application

• Current Assignee: Olympus Corporation, Terumo Kabushiki Kaisha (Terumo Corporation)
• Original Assignee: Olympus Corporation, Terumo Kabushiki Kaisha (Terumo Corporation)
• Inventors: MAEDA, Kazuya, KOHNO, Hiromasa
• Application Number:
  US13/869,066
• Publication Number:
  US 20130234045A1
• Time in Patent Office: Days
• US Class Current: 250/459.1
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6454 using an integrated detector array
• CPC Class Codes: G01N 2201/1211 for temperature

Abstract

A correction method of a fluorescence sensor includes a first detection signal acquiring step for acquiring, at a first temperature, a first detection signal using a fluorescence sensor having a temperature detecting function and a temperature adjusting function, a second detection signal acquiring step for acquiring, at a second temperature, a second detection signal when an analyte amount is the same as an analyte amount in the first detection signal acquiring step, a correction coefficient calculating step for calculating, on the basis of the first detection signal and the second detection signal, a correction coefficient for correcting the fluorescent light detection signal, and a correcting step for correcting subsequent detection signals using the correction coefficient and a temperature detection signal.

Claims

1. A correction method of a fluorescence sensor comprising:

a first detection signal acquiring step for acquiring, at a first temperature, a first detection signal using a fluorescence sensor including a light-emitting element configured to generate excitation light, an indicator layer configured to generate fluorescent light corresponding to the excitation light and an analyte amount, and a photoelectric conversion element configured to output a detection signal in which an excitation light detection signal due to the excitation light is superimposed on a fluorescent light detection signal due to the fluorescent light, the fluorescence sensor having a temperature detecting function for outputting a temperature detection signal and a temperature adjusting function;

a second detection signal acquiring step for acquiring, at a second temperature, a second detection signal when the analyte amount is the same as the analyte amount in the first detection signal acquiring step;

a correction coefficient calculating step for calculating, on the basis of the first detection signal and the second detection signal, a correction coefficient for correcting the fluorescent light detection signal; and

a correcting step for correcting subsequent detection signals using the correction coefficient and the temperature detection signal.

2. The correction method of the fluorescence sensor according to claim 1, wherein at least respective parts of the photoelectric conversion element, the light-emitting element that transmits the fluorescent light, and the indicator layer are formed in a same region on a substrate in this order.

3. The correction method of the fluorescence sensor according to claim 1, wherein the fluorescence sensor includes a temperature adjusting section having the temperature adjusting function.

4. The correction method of the fluorescence sensor according to claim 3, wherein the temperature adjusting section is a heater, the heater being a resistance heating type element.

5. The correction method of the fluorescence sensor according to claim 1, wherein the light-emitting element has the temperature adjusting function.

6. The correction method of the fluorescence sensor according to claim 1, wherein the photoelectric conversion element is a diode having the temperature adjusting function.

7. The correction method of the florescence sensor according to claim 1, wherein the photoelectric conversion element is a diode having the temperature detecting function.

8. A fluorescence sensor comprising:

a light-emitting element configured to generate excitation light;

an indicator layer configured to generate fluorescent light corresponding to the excitation light and an analyte amount; and

a photoelectric conversion element configured to output a detection signal in which an excitation light detection signal due to the excitation light is superimposed on a fluorescent light detection signal due to the fluorescent light,

wherein the fluorescence sensor has a temperature detecting function for outputting a temperature detection signal and a temperature adjusting function.

9. The fluorescence sensor according to claim 8, further comprising a resistance heating type element wound around the indicator layer, the resistance heating type element having the temperature adjusting function.

10. The fluorescence sensor according to claim 9, wherein a correction coefficient for calculating the fluorescent light detection signal is calculated on the basis of a plurality of the detection signals when the fluorescence sensor is adjusted to a plurality of temperatures by the temperature adjusting section, and subsequent detection signals are corrected using the correction coefficient.

11. The fluorescence sensor according to claim 10, wherein at least respective parts of the photoelectric conversion element, the light-emitting element that transmits the fluorescent light, and the indicator layer are formed in a same region on an upper side of a substrate in this order.

12. The fluorescence sensor according to claim 11, wherein the fluorescence sensor is a needle type sensor including a connector section that fits with a fitting section of a main body section arranged on an outside of a body, the needle type sensor measuring an analyte in the body.