POLYMERIC DEVICE SUITABLE FOR ULTRAVIOLET DETECTION

• US 20130240747A1
• Filed: 05/10/2013
• Published: 09/19/2013
• Est. Priority Date: 02/20/2007
• Status: Active Grant

• Current Assignee: Cytiva Sweden AB (Danaher Corporation)
• Original Assignee: GE Healthcare Bio-Sciences AB (GE Healthcare Technologies, Inc.)
• Inventors: EHRING, HANNO
• Granted Patent: US 9,404,862 B2
• Time in Patent Office: Days
• US Class Current: 250/373
• CPC Class Codes: A61L 2/08 Radiation
• CPC Class Codes: A61L 2202/11 Apparatus for generating biocidal substances, e.g. vaporisers, UV lamps
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/33 using ultraviolet light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/59 Transmissivity G01N21/25 takes precedence
• CPC Class Codes: G01N 2201/062 LED's
• CPC Class Codes: G01N 30/74 Optical detectors measurement of intensity, velocity, spectral content, polarisation, or phase of infrared, visible or ultraviolet light G01J
• CPC Class Codes: G01N 33/48707 by electrical means G01N33/49, G01N33/493 take precedence

Abstract

The present invention relates to a flow cell (10) comprising a fluid inlet (16) and a fluid outlet (18) separated by a sample flow-through chamber (12) comprising at least one UV-transparent window (22′), wherein the at least one UV-transparent window (22′) is made of a polymer material and has been subjected to Gamma radiation sterilisation. In one aspect, the flow cell is combustible.

Claims

1. A method for measuring UV absorption of a sample using a flow cell (10) comprising:

a fluid inlet (16) and a fluid outlet (18) separated by a sample flow-through chamber (12) comprising at least one UV-transparent window (22′

), wherein said at least one UV-transparent window (22′

) is made of a polymer material and has been subjected to Gamma radiation sterilisation.

2. The method of claim 1, wherein a dose of at least 10 kGy is used for said Gamma radiation sterilisation.

3. The method of claim 1, wherein said polymer material has a UV transmission in the range of between 1% and 40% per mm at a wavelength of 280 nm after being subjected to Gamma radiation sterilisation.

4. The method of claim 1, wherein the transmission of the polymer material after being subjected to Gamma radiation sterilisation does not vary by more than 10% during exposure for a period of 72 hours to UV-light of an intensity of approximately 0.6 μ

W/mm².

5. The method of claim 1, wherein the transmission of the polymer material after being subjected to Gamma radiation sterilisation does not vary by more than 10% during exposure for a period of 48 hours to UV-light of an intensity of approximately 0.6 μ

W/mm².

6. The method of claim 1, wherein the UV-transmission of the polymer material is greater than 2% and less than 30% per mm after being subjected to Gamma radiation sterilisation.

7. The method of claim 1, wherein the UV-transmission of the polymer material is greater than 5% and less than 20% per mm after being subjected to Gamma radiation sterilisation.

8. The method of claim 1, wherein the UV-transmission of the polymer material is greater than 12% and less than 18% per mm after being subjected to Gamma radiation sterilisation.

9. The method of claim 1, wherein a sealing means (30) is fitted outside the flow cell to provide a sealable flow cell (31).

10. The method of claim 9, wherein said sealing means is made of an opaque material so that essentially no UV radiation, visible radiation or near infrared radiation can pass through the material, said sealing means additionally comprising a UV radiation transmission window (44).

11. The method of claim 1, wherein it being combustible.

12. The method of claim 1, wherein the UV radiation is provided by one or more light emitting diodes.

13. A kit of parts comprising a flow cell (10) comprising a fluid inlet (16) and a fluid outlet (18) separated by a sample flow-through chamber (12) comprising at least one UV-transparent window (22′

), wherein said at least one UV-transparent window (22′

) is made of a polymer material and has been subjected to Gamma radiation sterilisation and a UV absorption detector which is open on one side.