Centrifuge

• US 20130244856A1
• Filed: 02/13/2013
• Published: 09/19/2013
• Est. Priority Date: 11/19/2010
• Status: Active Grant

• Current Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Original Assignee: Kensey Nash Corporation (DSM-Firmenich AG)
• Inventors: Nash, John E., Fisher, William T., Evans, Douglas G.
• Granted Patent: US 8,870,733 B2
• Time in Patent Office: Days
• US Class Current: 494/4
• CPC Class Codes: A61M 1/3693 using separation based on different densities of components, e.g. centrifuging
• CPC Class Codes: A61M 1/3696 with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
• CPC Class Codes: B01D 21/262 by using a centrifuge
• CPC Class Codes: B01D 21/34 Controlling the feed distribution; Controlling the liquid level ; Control of process parameters
• CPC Class Codes: B01D 2221/10 Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues apparatus for dental treatment A61C17/065
• CPC Class Codes: B04B 1/02 without inserted separating walls
• CPC Class Codes: B04B 1/14 with periodical discharge
• CPC Class Codes: B04B 1/18 controlled by the centrifugal force of an auxiliary liquid
• CPC Class Codes: B04B 11/04 Periodical feeding or discharging; Control arrangements therefor
• CPC Class Codes: B04B 2005/0485 with a displaceable piston in the centrifuge chamber
• CPC Class Codes: B04B 5/0407 for liquids contained in receptacles B04B5/0442 takes precedence
• CPC Class Codes: B04B 5/0442 with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
• CPC Class Codes: B04B 7/08 Rotary bowls centrifugal casting machines B22D

Abstract

Centrifuges are useful to, among other things, remove red blood cells from whole blood and retain platelets and other factors in a reduced volume of plasma. Platelet rich plasma (PRP) and or platelet poor plasma (PPP) can be obtained rapidly and is ready for immediate injection into the host. Embodiments may include valves, operated manually or automatically, to open ports that discharge the excess red blood cells and the excess plasma into separate receivers while retaining the platelets and other factors in the centrifuge chamber. High speeds used allow simple and small embodiments to be used at the patient'"'"'s side during surgical procedures. The embodiments can also be used for the separation of liquids or slurries in other fields such as, for example, the separation of pigments or lubricants.

Claims

1. A centrifuge for selectively concentrating and collecting constituents of a biologic liquid mixture, said constituents having differing specific gravities and being stratifiable in a centrifugal field produced by said centrifuge, said centrifuge comprising a disk-like separation chamber, a first receiving chamber and a second receiving chamber, said separation chamber having a central rotational axis, an end wall and a side wall tapering radially inward toward said central longitudinal axis from said end wall, said separation chamber being arranged to have said biologic liquid mixture introduced therein and to be rotated about said central longitudinal axis by a motor to produce said centrifugal field, whereupon said constituents of said biologic liquid mixture stratify into at least two concentric stratified constituent layers having an interface between immediately adjacent constituent layers, said first receiving chamber being coupled to said separation chamber for rotation therewith, said second receiving chamber also being coupled to said separation chamber for rotation therewith, said first receiving chamber being coupled to a first valve which when opened enables a first constituent layer to be automatically ejected from said separation chamber through said first valve into said first receiving chamber as a result of pressure built up by said centrifugal field, said second receiving chamber being coupled to a second valve which when opened enables a second constituent layer to be automatically ejected from said separation chamber through said second valve into said second receiving chamber as a result of pressure built up by said centrifugal field.

2. The centrifuge of claim 1, wherein said first receiving chamber is a hollow member disposed about said separation chamber.

3. The centrifuge of claim 1 wherein said first receiving chamber is made up of a plurality of linked chambers disposed about said separation chamber

4. The centrifuge of claim 1, wherein said second receiving chamber is a hollow member disposed about said separation chamber.

5. The centrifuge of claim 1 wherein said second receiving chamber is made up of a plurality of linked chambers disposed about said separation chamber

6. The centrifuge of claim 5 wherein said first receiving chamber is made up of a plurality of linked chambers disposed about said second receiving chamber.

7. The centrifuge of claim 1 wherein said centrifuge comprises a first passageway leading from said separation chamber to said first valve.

8. The centrifuge of claim 1 wherein said centrifuge comprises a second passageway leading from said separation chamber to said second valve.

9. The centrifuge of claim 1 wherein after the first constituent layer is located in said first receiving chamber and the second constituent layer is located in said second receiving chamber a residual constituent of the biologic liquid mixture remains in said separation chamber, the residual constituent being removable from said separation chamber through an access port.

10. The centrifuge of claim 9 wherein said access port is located in said end wall of said separation chamber.

11. The centrifuge of claim 10 wherein said access port is arranged to enable the biologic liquid mixture to be introduced into said separation chamber via said openable port.

12. The centrifuge of claim 11 wherein said access port comprises a piercable membrane

13. The centrifuge of claim 11 wherein said access port comprises a one-way valve.

14. The centrifuge of claim 1 wherein the biologic liquid mixture comprises blood and said separation chamber has a maximum diameter of approximately 8 cm and a height measured along said longitudinal axis of approximately 1 cm for a capacity of approximately 30 mL.

15. The centrifuge of claim 1 wherein said end wall includes at least a portion that is transparent to enable the constituent layer interface when located at a predetermined position in the receiving chamber to be visible by the operator of the centrifuge through said transparent portion of said end wall.

16. The centrifuge of claim 15 wherein at least a portion of said separation chamber which is disposed opposite to said transparent end wall portion provides a contrasting background to facilitate the operator of the centrifuge determining the existence of the constituent layer interface at the predetermined position.

17. The centrifuge of claim 1 wherein said separation chamber, said first and second receiving chambers and said first and second valves comprise an integral unit, said integral unit being arranged to be coupled to a base unit including a motor for effecting the rotation of said separating chamber.

18. The centrifuge of claim 17 wherein said integral unit is disposable.

19. The centrifuge of claim 1 wherein said centrifuge comprises a plate member having a first port in fluid communication with said separation chamber and a first wall portion forming a wall of said first receiving chamber, said first wall portion having a third port in fluid communication with said first chamber, and wherein said first valve comprises a first member adapted for rotation with said separation chamber and movable relative to said first wall portion, said first member comprising a first passageway which when said first member is moved relative to said first wall portion to an open position causes said first passageway to bridge said first and third ports, whereupon the first constituent layer automatically flows through said communicating first and third ports into said first receiving chamber.

20. The centrifuge of claim 1 wherein said centrifuge comprises a plate member having a second port in fluid communication with said separation chamber and a second wall portion forming a wall of said second receiving chamber, said second wall portion having a fourth port in fluid communication with said second chamber, and wherein said second valve comprises a second member adapted for rotation with said separation chamber and movable relative to said second wall portion, said second member comprising a second passageway which when said second member is moved relative to said second wall portion to an open position causes said second passageway to bridge said second and fourth ports, whereupon the second constituent layer automatically flows through said communicating second and fourth ports into said second receiving chamber.

21. The centrifuge of claim 19 wherein said centrifuge comprises a plate member having a second port in fluid communication with said separation chamber and a second wall portion forming a wall of said second receiving chamber, said second wall portion having a fourth port in fluid communication with said second chamber, and wherein said second valve comprises a second member adapted for rotation with said separation chamber and movable relative to said second wall portion, said second member comprising a second passageway which when said second member is moved relative to said second wall portion to an open position causes said second passageway to bridge said second and fourth ports, whereupon the second constituent layer automatically flows through said communicating second and fourth ports into said second receiving chamber.

22. The centrifuge of claim 21 wherein said first member of said first valve and said second member of said second valve are coupled together for pivoting with respect to said central longitudinal axis, said first member of said first valve being arranged to be pivoted in a first rotational direction to bring said first member to said open position, said second member of said second valve being arranged to be pivoted in a second rotational direction to bring said second member to said open position, said second rotational direction being opposite said first rotational direction.

23. The centrifuge of claim 21 wherein said first port is in fluid communication with said separation chamber at a first radial distance from said central longitudinal axis and said second port is in fluid communication with said separation chamber at a second radial distance from said central longitudinal axis, said first radial distance being greater than said second radial distance.