CONFOCAL PHOTOACOUSTIC MICROSCOPY WITH OPTICAL LATERAL RESOLUTION
First Claim
1. A method for determining a ratio of hemoglobin absorption within a biological tissue using a handheld device, said method comprising:
- focusing at least one light pulse into a predetermined area inside an object using at least one excitation wavelength, wherein a fluorescence signal is based on at least a local excitation optical fluence and properties of fluorophores in the predetermined area;
receiving a photoacoustic signal emitted by the object in response to the at least one light pulse, the photoacoustic signal being a product of the local excitation optical fluence, an optical absorption coefficient of hemoglobin, and an acoustic detection sensitivity;
determining the ratio of hemoglobin absorption based on a hemoglobin absorption coefficient that is based at least partially on the fluorescence signal and the photoacoustic signal.
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Abstract
A method for determining a ratio of hemoglobin absorption within a biological tissue using a handheld device is provided. The method includes focusing at least one light pulse into a predetermined area inside an object using at least one excitation wavelength, wherein a fluorescence signal is based on at least a local excitation optical fluence and properties of fluorophores in the predetermined area. The method further includes receiving a photoacoustic signal emitted by the object in response to the at least one light pulse, the photoacoustic signal being a product of the local excitation optical fluence, an optical absorption coefficient of hemoglobin, and an acoustic detection sensitivity. The method further includes determining the ratio of hemoglobin absorption based on a hemoglobin absorption coefficient that is based at least partially on the fluorescence signal and the photoacoustic signal.
22 Citations
20 Claims
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1. A method for determining a ratio of hemoglobin absorption within a biological tissue using a handheld device, said method comprising:
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focusing at least one light pulse into a predetermined area inside an object using at least one excitation wavelength, wherein a fluorescence signal is based on at least a local excitation optical fluence and properties of fluorophores in the predetermined area; receiving a photoacoustic signal emitted by the object in response to the at least one light pulse, the photoacoustic signal being a product of the local excitation optical fluence, an optical absorption coefficient of hemoglobin, and an acoustic detection sensitivity; determining the ratio of hemoglobin absorption based on a hemoglobin absorption coefficient that is based at least partially on the fluorescence signal and the photoacoustic signal. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for determining an oxygen metabolic rate (MRO2) within a biological tissue using a handheld device, said method comprising:
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focusing at least one light pulse into a predetermined area inside an object; receiving acoustic waves emitted by the object in response to the at least one light pulse; and determining the MRO2 based on an area of an incoming vessel within the predetermined area, a mean flow velocity of blood in an incoming vessel, and a total concentration of hemoglobin, wherein the area of the incoming vessel and the mean flow velocity may are based on measurements obtained by one of ultrasound imaging, optical coherence tomography, and flow imaging technologies, and wherein the total concentration of hemoglobin is based on measurements obtained by the at least one light pulse. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
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19. An apparatus for determining a ratio of hemoglobin absorption within a biological tissue, said apparatus comprising:
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a focusing assembly configured to focus at least one light pulse into a predetermined area inside an object using at least one excitation wavelength, wherein a fluorescence signal is based on at least a local excitation optical fluence and properties of fluorophores in the predetermined area; an ultrasonic transducer configured to receive a photoacoustic signal emitted by the object in response to the at least one light pulse, the photoacoustic signal being a product of the local excitation optical fluence, an optical absorption coefficient of hemoglobin, and an acoustic detection sensitivity; and a processor configured to determine the ratio of hemoglobin absorption based on a hemoglobin absorption coefficient that is based at least partially on the fluorescence signal and the photoacoustic signal. - View Dependent Claims (20)
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Specification