×

DNA SEQUENCING BY SYNTHESIS USING MODIFIED NUCLEOTIDES AND NANOPORE DETECTION

  • US 20130264207A1
  • Filed: 12/16/2011
  • Published: 10/10/2013
  • Est. Priority Date: 12/17/2010
  • Status: Active Grant
First Claim
Patent Images

1. A method for determining the nucleotide sequence of a single-stranded DNA comprising:

  • (a) contacting the single-stranded DNA, wherein the single-stranded DNA is in an electrolyte solution in contact with a nanopore in a membrane and wherein the single-stranded DNA has a primer hybridized to a portion thereof, with a DNA polymerase and at least four deoxyribonucleotide polyphosphate (dNPP) analogues under conditions permitting the DNA polymerase to catalyze incorporation of one of the dNPP analogues into the primer if it is complementary to the nucleotide residue of the single-stranded DNA which is immediately 5′

    to a nucleotide residue of the single-stranded DNA hybridized to the 3′

    terminal nucleotide residue of the primer, so as to form a DNA extension product, wherein each of the four dNPP analogues has the structure;

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×