BIOLOGICAL SAMPLE FILTERING SYSTEM AND METHOD FOR FILTERING BIOLOGICAL SAMPLES

• US 20130264286A1
• Filed: 03/18/2013
• Published: 10/10/2013
• Est. Priority Date: 04/06/2012
• Status: Abandoned Application

• Current Assignee: Cells Scientific Corporation
• Original Assignee: Cells Scientific Corporation
• Inventors: Tai, Chi-Sheng
• Application Number:
  US13/845,872
• Publication Number:
  US 20130264286A1
• Time in Patent Office: Days
• US Class Current: 210/636
• CPC Class Codes: B01D 65/027 Cleaning of other parts of the apparatus than the membrane
• CPC Class Codes: B01L 2200/146 Employing pressure sensors
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: C12M 47/02 Separating microorganisms from the culture medium; Concentration of biomass separating microorganisms from their culture media C12N1/02

Abstract

A biological sample filtering system for filtering biological liquid samples includes: a sample-mounting box; a plurality of column units disposed in sample wells in the sample-mounting box; a filtrate-collecting container; a control valve connected to the sample-mounting box and the filtrate-collecting container; and a vacuum pump connected to the filtrate-collecting container for generating a vacuum in the box chamber that permits a liquid in the biological liquid samples to be flushed from the column units into the filtrate-collecting container.

Claims

1. A biological sample filtering system for filtering biological liquid samples containing a liquid, impurities, and substances to be separated from the liquid and the impurities, said biological sample filtering system comprising:

a sample-mounting box that defines an enclosed box chamber and a fluid outlet in fluid communication with said box chamber and that has an upper manifold formed with a plurality of sample wells in fluid communication with said box chamber;

a plurality of column units, each of which is disposed in a respective one of said sample wells, each of which is adapted to receive one of the biological liquid samples, and each of which has a tube member and a membrane member mounted in said tube member for filtering the substances in the biological liquid samples, said tube member being in fluid communication with the respective one of said sample wells;

a filtrate-collecting container defining a filtrate-collecting space and having a fluid inlet and an air outlet, said filtrate-collecting space being in fluid communication with said fluid inlet and said air outlet;

a control valve disposed between and connected to said fluid outlet of said sample-mounting box and said fluid inlet of said filtrate-collecting container for controlling fluid communication between said box chamber and said filtrate-collecting space; and

a vacuum pump connected to said air outlet of said filtrate-collecting container and capable of generating a vacuum in said filtrate-collecting space when said control valve is closed so as to generate a vacuum in said box chamber when said control valve is subsequently opened and to permit the liquid together with the impurities in the biological liquid samples to be flushed from said column units through said membrane members and said box chamber and into said filtrate-collecting space.

2. The biological sample filtering system of claim 1, wherein the volume ratio of the volume of said filtrate-collecting space to the volume of said box chamber is greater than 4:

1.

3. The biological sample filtering system of claim 1, further comprising a pressure detector and a valve controller, said pressure detector being connected to said filtrate-collecting container for detecting the pressure in said filtrate-collecting space, said valve controller being connected to said pressure detector and said control valve so as to receive a pressure signal, that corresponds to the pressure in said filtrate-collecting space, from said pressure detector and so as to control opening of said control valve based on the pressure signal.

4. A method of filtering biological liquid samples containing a liquid, impurities and substances to be separated from the liquid and the impurities using a biological sample filtering system that includes a sample-mounting box, a filtrate-collecting container, a control valve disposed between and connected to the sample-mounting box and the filtrate-collecting container, and a vacuum pump connected to the filtrate-collecting container, the sample-mounting box defining a box chamber and a plurality of sample wells in fluid communication with the box chamber, the filtrate-collecting container defining a filtrate-collecting space, the method comprising:

(a) disposing a plurality of column units in the sample wells, respectively, each of the column units having a tube member and a membrane member mounted in the tube member for filtering the substances in the biological liquid samples;

(b) prevacuuming the filtrate-collecting space to a preset pressure while the control valve is closed to prevent fluid communication between the box chamber and the filtrate-collecting space; and

(c) opening the control valve after step (b) to permit fluid communication between the box chamber and the filtrate-collecting space so as to generate instantly a vacuum in the box chamber that permits the liquid together with the impurities in the biological liquid samples to be flushed from the column units through the membrane members and the box chamber and into the filtrate-collecting space.

5. The method of claim 4, wherein, in step (c), the pressure in the box chamber is reduced from the atmospheric pressure to less than 160 mmHg immediately after the control valve is opened so as to generate the flushing action.

6. The method of claim 4, wherein the volume ratio of the volume of the filtrate-collecting space to the volume of the box chamber is greater than 4:

1.