SERUM-FREE MEDIA AND THEIR USES FOR CHONDROCYTE EXPANSION

• US 20130273010A1
• Filed: 04/16/2013
• Published: 10/17/2013
• Est. Priority Date: 06/20/2006
• Status: Abandoned Application

• Current Assignee: Genzyme Corporation (Sanofi )
• Original Assignee: Genzyme Corporation (Sanofi )
• Inventors: DUGUAY, Stephen, SEYMOUR, Barbara
• Application Number:
  US13/864,149
• Publication Number:
  US 20130273010A1
• Time in Patent Office: Days
• US Class Current: 424/93.7
• CPC Class Codes: A61K 35/32 Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: C12N 2500/36 Lipids
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2501/135 Platelet-derived growth factor [PDGF]
• CPC Class Codes: C12N 2501/2306 Interleukin-6 (IL-6)
• CPC Class Codes: C12N 2501/235 Leukemia inhibitory factor [LIF]
• CPC Class Codes: C12N 2501/237 Oncostatin M [OSM]
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 2501/58 Adhesion molecules, e.g. ICAM, VCAM, CD18 (ligand), CD11 (ligand), CD49 (ligand)
• CPC Class Codes: C12N 2533/74 Alginate
• CPC Class Codes: C12N 5/0655 Chondrocytes; Cartilage

Abstract

The present invention provides defined serum-free cell culture media useful in culturing fibroblasts, especially articular chondrocytes, that avoid problems inherent in the use of serum-containing media. The defined media comprise platelet-derived growth factor (PDGF), chemically defined lipids, oncostatin M (OSM), interleukin-6 (IL-6), leukemia inhibitory factor (LIF), or combinations of these compounds. In another aspect, the present invention also provides tissue culture methods that comprise incubating chondrocytes in the defined serum-free media. The methods enhance attachment and proliferative expansion of chondrocytes seeded at low density while maintaining their redifferentiation potential.

Claims

1. A culture medium comprising a basal medium supplemented with substantially pure oncostatin M (OSM).

2. The culture medium of claim 1, wherein the basal medium is additionally supplemented with substantially pure interleukin-6 (IL-6).

3. The culture medium of claim 1, wherein the basal medium is additionally supplemented with substantially pure leukemia inhibitory factor (LIF).

4. The culture medium of claim 1, wherein the basal medium is additionally supplemented with substantially pure IL-6 and substantially pure LIF.

5. The culture medium of claim 1, wherein the basal medium is cDRF.

6. The culture medium of claim 1, wherein the basal medium is additionally supplemented with substantially pure platelet-derived growth factor (PDGF).

7. The culture medium of claim 1, wherein the basal medium is additionally supplemented with one or more lipids selected from the group consisting of stearic acid, myristic acid, oleic acid, linoleic acid, palmitic acid, palmitoleic acid, arachidonic acid, linolenic acid, cholesterol, and alpha-tocopherol acetate.

8. The culture medium of claim 1, wherein the basal medium is cDRF and is additionally supplemented with PDGF and a chemically defined lipid mixture (CDLM).

9. The culture medium of claim 1, wherein the basal medium is cDRFm and is additionally supplemented with PDGF and CDLM.

10. The culture medium of claim 2, wherein the basal medium is cDRFm and is additionally supplemented with PDGF and CDLM.

11. The culture medium of claim 1, wherein the culture medium is not supplemented with substantially pure jagged 1 (JAG1) and/or substantially pure interleukin-13 (IL-13).

12. The culture medium of claim 1, wherein OSM is present at a concentration from 0.01 ng/ml to 10 ng/ml in the culture medium.

13. The culture medium of claim 2, wherein each one of OSM and IL-6 is present at a concentration from 0.01 ng/ml to 10 ng/ml in the culture medium.

14. The culture medium of claim 3, wherein each one of OSM and LIF is present at a concentration from 0.01 ng/ml to 10 ng/ml in the culture medium.

15. The culture medium of claim 4, wherein each one of OSM, IL-6, and LIF is present at a concentration from 0.01 ng/ml to 10 ng/ml in the culture medium.

16. The culture medium of claim 1, wherein the culture medium is serum-free.

17. The culture medium of claim 1, wherein the culture medium further comprises serum.

50. A chondrocyte cultured using the culture medium of claim 1.

53. A method of treating a cartilage defect in a subject, comprising:

(a) culturing a chondrocyte using the method of claim 1; and

(b) administering the chondrocyte to the subject.

56. A composition comprising a chondrocyte and the culture medium of claim 1.

18. A culture medium comprising:

(a) cDRFm;

(b) 0.1-100 ng/ml PDGF;

(c) 0.05-5% CDLM;

(d) 0.01-10 ng/ml OSM; and

(e) 0.01-10 ng/ml IL-6.

19. A method of culturing cells, comprising the step of incubating the cell with a culture medium comprising a basal medium supplemented with substantially pure OSM.

20. The method of claim 19, wherein the basal medium is additionally supplemented with substantially pure IL-6.

21. The method of claim 19, wherein the basal medium is additionally supplemented with substantially pure LIF.

22. The method of claim 19, wherein the basal medium is additionally supplemented with substantially pure IL-6 and substantially pure LIF.

23. The method of claim 19, wherein the basal medium is cDRF.

24. The method of claim 19, wherein the basal medium is additionally supplemented with substantially pure PDGF.

25. The method of claim 19, wherein the basal medium is additionally supplemented with one or more lipids selected from the group consisting of stearic acid, myristic acid, oleic acid, linoleic acid, palmitic acid, palmitoleic acid, arachidonic acid, linolenic acid, cholesterol, and alpha-tocopherol acetate.

26. The method of claim 19, wherein the basal medium is cDRF and is additionally supplemented with PDGF and CDLM.

27. The method of claim 19, wherein the basal medium is cDRFm and is additionally supplemented with PDGF and CDLM.

28. The method of claim 20, wherein the basal medium is cDRFm and is additionally supplemented with PDGF and CDLM.

29. The method of claim 19, wherein the culture medium is not supplemented with substantially pure JAG1 and/or substantially pure IL-13.

30. The method of claim 19, wherein OSM is present at a concentration from 0.01 ng/ml to 10 ng/ml in the culture medium.

31. The method of claim 20, wherein each one of OSM and IL-6 is present at a concentration from 0.01 ng/ml to 10 ng/ml in the culture medium.

32. The method of claim 21, wherein each one of OSM and LIF is present at a concentration from 0.01 ng/ml to 10 ng/ml in the culture medium.

33. The method of claim 22, wherein each one of OSM, IL-6, and LIF is present at a concentration from 0.01 ng/ml to 10 ng/ml in the culture medium.

34. The method of claim 19, wherein the culture medium is serum-free.

35. The method of claim 19, wherein the culture medium further comprises serum.

36. The method of claim 19, wherein the cells are chondrocytes.

37. The method of claim 36, wherein chondrocytes are de-differentiated.

38. The method of claim 36, wherein the chondrocytes are derived from mesenchymal stem cells.

39. The method of claim 36, wherein the chondrocytes are human chondrocytes.

40. The method of claim 36, wherein the chondrocytes are human articular chondrocytes.

41. The method of claim 36, wherein the chondrocytes are primary.

42. The method of claim 19, further comprising the step of passaging the cells.

43. The method of claim 42, wherein the cell is passaged by incubating the cells with a solution comprising a chelating agent.

44. The method of claim 43, wherein the chelating agent is EDTA.

45. The method of claim 44, wherein EDTA is present in the solution at a concentration from 0.1 mM to 1 mM.

46. The method of claim 42, wherein the cells are passaged by incubating the cells with a solution containing less than 325 units/ml trypsin.

47. The method of claim 46, wherein the solution contains from 0.1 mM to 1 mM EDTA.

48. The method of claim 19, wherein the cells are seeded at a density less than 20,000 cells/cm².

51. A chondrocyte cultured using the method of claim 19.

54. A method of treating a cartilage defect in a subject, comprising:

(a) culturing a chondrocyte using the method of claim 19; and

(b) administering the chondrocyte to the subject.

49. A method for culturing a chondrocyte, comprising the step of incubating the chondrocyte with a culture medium comprising:

(a) cDRFm;

(b) 0.1-100 ng/ml PDGF;

(c) 0.05-5% CDLM;

(d) 0.01-10 ng/ml OSM; and

(e) 0.01-10 ng/ml IL-6.

52. A chondrocyte cultured using the method of claim 49.

55. A method of treating a cartilage defect in a subject, comprising:

(a) culturing a chondrocyte using the method of claim 49; and

(b) administering the chondrocyte to the subject.

57. A composition comprising a chondrocyte and a culture medium comprising:

(a) cDRFm;

(b) 0.1-100 ng/ml PDGF;

(c) 0.05-5% CDLM;

(d) 0.01-10 ng/ml OSM; and

(e) 0.01-10 ng/ml IL-6.

58. A culture medium comprising a basal medium supplemented with:

(a) one or both of substantially pure PDGF and CDLM; and

(b) one or more of substantially pure OSM, substantially pure IL-6, and substantially pure LIF.

59. The culture medium of claim 58, wherein the basal medium is cDRF.

60. The culture medium of claim 58, wherein the basal medium is cDRFm.

61. The culture medium of claim 58, wherein the basal medium is supplemented with substantially pure PDGF and CDLM.