INSULIN PRODUCING CELLS DERIVED FROM PLURIPOTENT STEM CELLS
First Claim
1. A method of generating islet cells or islet progenitor cells from pluripotent stem cells, the method comprising:
- (a) culturing the pluripotent stem cells in a differentiation medium so as to differentiate the pluripotent stem cells into endoderm cells; and
(b) culturing said endoderm cells in a medium comprising at least one growth factor, a cAMP inducer and retinoic acid (RA), said at least one growth factor being selected from the group consisting of FGF10, bFGF and FGF7 so as to generate further differentiated cells; and
(c) culturing said further differentiated cells in a medium comprising a maturation factor selected from the group consisting of nicotinamide, GLP-1 and exendin 4, thereby generating islet cells or islet progenitor cells from pluripotent stem cells;
(d) contacting said islet cells or islet progenitor cells with an agent that binds to EpCAM; and
(e) selecting cells which bind to said agent.
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Abstract
A method of generating islet cells from pluripotent stem cells is disclosed. The method comprises: (a) culturing the pluripotent stem cells in a differentiation medium so as to differentiate the pluripotent stem cells into endoderm cells; and (b) culturing the endoderm cells in a medium comprising at least one growth factor, a cAMP inducer and retinoic acid (RA), said at least one growth factor being selected from the group consisting of FGF10, bFGF and FGF7 so as to generate further differentiated cells; and (c) culturing the further differentiated cells in a medium comprising a maturation factor selected from the group consisting of nicotinamide, GLP-1 and exendin 4, thereby generating islet cells from pluripotent stem cells. Further methods of generating islet cells are also disclosed, isolated cell populations comprising same and uses thereof.
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Citations
39 Claims
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1. A method of generating islet cells or islet progenitor cells from pluripotent stem cells, the method comprising:
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(a) culturing the pluripotent stem cells in a differentiation medium so as to differentiate the pluripotent stem cells into endoderm cells; and (b) culturing said endoderm cells in a medium comprising at least one growth factor, a cAMP inducer and retinoic acid (RA), said at least one growth factor being selected from the group consisting of FGF10, bFGF and FGF7 so as to generate further differentiated cells; and (c) culturing said further differentiated cells in a medium comprising a maturation factor selected from the group consisting of nicotinamide, GLP-1 and exendin 4, thereby generating islet cells or islet progenitor cells from pluripotent stem cells; (d) contacting said islet cells or islet progenitor cells with an agent that binds to EpCAM; and (e) selecting cells which bind to said agent. - View Dependent Claims (4, 5, 6, 7, 9, 11, 12, 13, 15, 17, 18, 19, 21, 22, 23, 24, 25, 29, 30, 32, 35, 38, 39)
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Specification