METHODS FOR DIAGNOSING IMPENDING DIARRHEA

• US 20130280724A1
• Filed: 03/27/2013
• Published: 10/24/2013
• Est. Priority Date: 04/11/2012
• Status: Abandoned Application

• Current Assignee: Nestec SA (Nestle SA)
• Original Assignee: Nestec SA (Nestle SA)
• Inventors: Xu, Hui, Ramadan, Ziad S., Laftamme, Dorothy P.
• Application Number:
  US13/851,421
• Publication Number:
  US 20130280724A1
• Time in Patent Office: Days
• US Class Current: 435/6.12
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/689 for bacteria
• CPC Class Codes: G01N 2800/06 Gastro-intestinal diseases
• CPC Class Codes: G01N 33/56911 Bacteria

Abstract

The invention provides methods for diagnosing impending diarrhea in an asymptomatic animal. The methods comprise obtaining a fecal sample from the animal; identifying one or more microorganisms in the fecal sample; and diagnosing impending diarrhea in the animal based on the presence or absence of one or more of the microorganisms.

Claims

1. A method for diagnosing impending diarrhea in an asymptomatic animal comprising:

obtaining a feral sample from the animal;

identifying one or more microorganisms in the fecal sample, and

diagnosing impending diarrhea in the animal based on (1) the presence of one or microorganisms selected from a first group consisting of Alistipes putredinis, Anaerobiospirillum succiniciproducens, Bacteroides coprocola, Clostridium perfringens, Clostridium spiroforme, Enterobacter hormaechei, Faecalibacterium prausnitzii, Helicobacter muridarum, Lactobacillus helveticus, Lactobacillus saerimneri. Bulleidia p_—

1630_c5, Parabacterides distasonis, Ruminococcus gnavus, Ruminococcus torques, Streptococcus minor, and Streptococcus suis, (2) the absence of one or more of the microorganisms selected from a second group consisting of Acidaminococcus fermentans, Bacteroides coprophilus, Campylobacter upsaliensis, Clostridium hiranonis, Clostridium orbiseindens, Collinsella aerofaciens, Eubacterium dolichum, Megasphaera elsdenii, and Prevotella copri, or a combination thereof, or (3) a combination thereof.

2. The method of claim 1 wherein the animal is diagnosed to have impending diarrhea if (1) two, three, four, five or more microorganisms selected from a first group consisting of Alistipes putredinis, Anaerobiospirillum succiniciproducens, Bacteroides coprocola, Clostridium perfringens, Clostridium spiroforme, Enterobacter hormaechei, Faecalibacterium prausnitzii, Helicobacter muridarum, Lactobacillus helveticus, Lactobacillus saerimneri, Bulleidia p_—

1630_c5, Parabacterides distasonis, Ruminococcus gnavus, Ruminococcus torques, Streptococcus minor, and Streptococcus suis are present in the fecal sample, (2) two, three, four, five or more microorganisms selected from a second group consisting of Acidaminococcus fermentans, Bacteroides coprophilus, Campylobacter upsaliensis, Clostridium hiranonis, Clostridium orbiscindens, aerofaciens, Eubacterium dolichum, Megasphaera elsdenii, and Prevotella copri are absent from the fecal sample, or 3 combinations thereof.

3. The method of claim 1 wherein the animal is diagnosed to have impending diarrhea if (1) at least three microorganisms selected from a first group consisting of Alistipes putredinis, Anaerobiospirillum succiniciproducens, Bacteroides coprocola, Clostridium perfringens, Clostridium spiroforme, Enterobacter hormaechei, Faecalibacterium prausnitzii, Helicobacter muridarum, Lactobacillus helveticus, Lactobacillus saerimneri. Bulleidia p_—

1630_c5, Parabacterides distasonis, Ruminococcus gnavus, Ruminococcus torques, Streptococcus minor, and Streptococcus suis are present in the fecal sample, (2) at least three microorganisms selected from a second group consisting of Acidaminococcus fermentans, Bacteroides coprophilus, Campylobacter upsaliensis, Clostridium hiranonis, Clostridium orbiscindens, Collinsella aerofaciens, Eubacterium dolichum, Megasphaera elsdenii, and Prevotella copri are absent from the fecal sample, or (3) combinations thereof.

4. The method of claim 1 wherein the microorganisms are selected from the first group consisting of Alistipes putredinis, Anaerobiospirillum succiniciproducens, Bacteroides coprocola, Clostridium perfringens, Clostridium spiroforme, Enterobacter hormaechei, Faecalibacterium prausnitzii, Helicobacter muridarum, Lactobacillus helveticus, Lactobacillus saerimneri. Bulleidia p_—

1630_c5, Parabacterides distasonis, Ruminococcus gnavus, Ruminococcus torques, Streptococcus minor, and Streptococcus suis.

5. The method of claim 1 wherein the microorganisms are selected from the first group consisting of Bacteroides coprocola, Clostridium perfringens, Clostridium spiroforme, Enterobacter hormaechei, Faecalibacterium prausnitzii. Helicobacter muridarum, Lactobacillus helveticus, Bulleidia p_—

1630_c5, Ruminococcus gnavus, Ruminococcus torques, Streptococcus minor, and Streptococcus suis.

6. The method of claim 1 wherein the microorganisms are selected from the first group consisting of Clostridium spiroforme and Streptococcus suis.

7. The method of claim 1 wherein the microorganisms are selected from the second group consisting of Acidaminococcus fermentans, Bacteroides coprophilus, Campylobacter upsaliensis, Clostridium hiranonis, Clostridium orbiscindens, Collinsella aerofaciens, Eubacterium dolichum, Megasphaera elsdenii, and Prevotella copri.

8. The method of claim 1 wherein the microorganisms are selected from the second group consisting of Acidaminococcus fermentans, Campylobacter upsaliensis, Clostridium orbiscindens, Megasphaera elsdenii, and Prevotella copri.

9. The method of claim 1 wherein the microorganisms are selected from the second group consisting of Acidaminococcus fermentans and Campylobacter upsaliensis.

10. The method of claim 1 wherein the animal is a feline.