Comprehensive Methylome Map of Myeloid and Lymphoid Commitment from Hematopoietic Proenitors
First Claim
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1. A method of identifying the differentiation potential of a cell comprising:
- comparing the methylation status of one or more nucleic acid sequences of a cell to a known methylation status of the one or more nucleic acid sequences of a reference progenitor cell, wherein a similarity or a difference in methylation status between the cell and the reference cell is indicative of the differentiation potential of the cell.
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Abstract
Provided herein are differentially methylated regions (DMRs) of multipotent progenitor cells (MPPs) and oligopotent progenitor cells and methods of use thereof. The invention provides methods for detecting and analyzing alterations in the methylation status of DMRs in such progenitor cells as well as methods for differentiating such cells.
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Citations
57 Claims
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1. A method of identifying the differentiation potential of a cell comprising:
comparing the methylation status of one or more nucleic acid sequences of a cell to a known methylation status of the one or more nucleic acid sequences of a reference progenitor cell, wherein a similarity or a difference in methylation status between the cell and the reference cell is indicative of the differentiation potential of the cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 55, 56, 57)
- 10. A method of modifying the lineage restriction of a partially or terminally differentiated myeloid or lymphoid cell comprising contacting a partially or terminally differentiated myeloid or lymphoid cell with an agent which alters regulation of the expression or expression product of a gene known to be associated with the differentiation potential of the cell, thereby modifying the lineage restriction of the cell.
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19-21. -21. (canceled)
- 22. A method of inducing myeloid differentiation of a progenitor cell, comprising contacting a progenitor cell with a demethylating agent, thereby inducing myeloid differentiation of the progenitor cell.
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28. (canceled)
- 29. A method of differentiating a progenitor cell comprising contacting a progenitor cell with an agent that alters regulation of the expression or expression product of a gene known to be associated with the differentiation potential of the cell, thereby differentiating the progenitor cell.
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38-40. -40. (canceled)
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42-45. -45. (canceled)
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46. A method of characterizing the methylation status of the nucleic acid of a cell, comprising:
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hybridizing labeled and digested nucleic acid of a cell to a DNA microarray comprising at least 2000 nucleic acid sequences; determining a pattern of methylation from the hybridizing of (a), thereby characterizing the methylation status for the cell, wherein the one or more nucleic acid sequences are selected from the Tables 2a to 2h, Tables 3a to 3h, FIGS. 1 , 3, 5, 8, 9, 10 and any combination thereof.- View Dependent Claims (47, 48, 50)
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49. (canceled)
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51. A plurality of nucleic acid sequences, selected from the group consisting of the differentially methylated region (DMR) sequences as set forth in Tables 2a to 2h,
FIGS. 1 , 3, 5, 8, 9, 10, and any combination thereof.
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53-54. -54. (canceled)
Specification