NUCLEIC ACID CONSTRUCT AND METHOD OF PREPARING NANOPARTICLE USING THE SAME

• US 20130310548A1
• Filed: 02/20/2013
• Published: 11/21/2013
• Est. Priority Date: 05/21/2012
• Status: Active Grant

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: PARK, No-kyoung
• Granted Patent: US 9,334,301 B2
• Time in Patent Office: Days
• US Class Current: 536/23.1
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C07H 23/00 Compounds containing boron, silicon, or a metal, e.g. chelates, vitamin B12 esters with inorganic acids C07H11/00; metal salts, see parent compounds

Abstract

A nucleic acid construct, a method of preparing a nanoparticle by using the nucleic acid construct, and a nanoparticle and nanoparticle complex prepared using the method. Various types of metal nanoparticles may be efficiently prepared using the template for preparing a nanoparticle and the nanoparticle prepared using the template.

Claims

1. A nanoparticle comprising a metal ion or a metal precursor, and a nucleic acid construct,

wherein the nucleic acid construct comprises at least two single-stranded nucleic acids, each of the at least two single-stranded nucleic acids comprising at least ten nucleotides and at least two adjacent segments, wherein each of the entire segments is complementary to one of the remainder segments, and wherein the segments are hybridized to their respective substantially complementary segments so that the nucleic acid construct comprises a plurality of double-stranded segments;

and

wherein the metal ion or metal precursor is bound to a phosphate backbone of the nucleic acid construct or to a base of the nucleic acid construct.

2. The nanoparticle of claim 1, wherein a metal of the metal ion or the metal precursor is selected from the group consisting of gold, silver, copper, platinum, palladium, and combinations thereof.

3. The nanoparticle of claim 1, wherein the nucleic acid construct comprises 2 to 10 single-stranded nucleic acids.

4. The nanoparticle of claim 1, wherein each of the at least two single-stranded nucleic acids comprises 2 to 5 segments.

5. The nanoparticle of claim 1, wherein the nucleic acid construct comprises at least one cross-linking or intercalating agent between two nucleic acid strands where segments are substantially complementarily bound to each other.

6. The nanoparticle of claim 1, wherein the adjacent segments do not comprise a linker, or further comprise a linker consisting of 1 to 50 nucleotides that do not complementarily bind to any one of the at least two single-stranded nucleic acids.

7. The nanoparticle of claim 1, wherein the nucleic acid construct comprises X-shaped, Y-shaped, liner, or a dumbbell-shaped double stranded segments.

8. The nanoparticle of claim 1, wherein the metal ion or the metal precursor forms a metal layer which is bound to a phosphate backbone of the nucleic acid construct or to a base of the nucleic acid construct.

9. A nanoparticle complex comprising at least two of the nanoparticles of claim 1, wherein an end of one of the at least two of the nanoparticles is bound to an end of the other thereof.

10. The nanoparticle complex of claim 9, wherein the nucleic acids are selected from the group consisting of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), peptide nucleic acid (PNA), locked nucleic acid (LNA), and analogs thereof.

11. A method of preparing a nanoparticle, the method comprising:

mixing at least two single-stranded nucleic acids each comprising at least two adjacent segments, wherein each of the entire segments is complementary to one of the remainder segments, to give a nucleic acid construct in which the segments are hybridized to their respective substantially complementary segments, and wherein the resulting nucleic acid construct comprises a plurality of double-stranded segments;

adding an intercalator to a resultant of the hybridization to allow the intercalator be intercalated into the double-stranded segments;

contacting the nucleic acid construct with a metal ion; and

subjecting the metal ion to reduction.

12. The method of claim 11, wherein the intercalator is selected from the group consisting of psoralen, ethidium bromide, proflavine, daunomycin, doxorubicin, thalidomide, YOYO-1, TOTO-1, YOYO-3, TOTO-3, POPO-1, BOBO-1, POPO-3, BOBO-3, LOLO-1, JOJO-1, cyanine dimers, YO-PRO-1, TO-PRO-1, YO-PRO-3, TO-PRO-3, TO-PRO-5, PO-PRO-1, BO-PRO-1, PO-PRO-3, BO-PRO-3, LO-PRO-1, JO-PRO-1, cyanine monomers, ethidium homodimer-1, ethidium homodimer-2, ethidium derivatives, acridine, acridine orange, acridine derivatives, ethidium-acridine heterodimer, ethidium monoazide, propidium iodide, SYTO dyes, SYBR Green 1, SYBR dyes, Pico Green, SYTOX dyes, and 7-aminoactinomycin D.

13. The method of claim 11, wherein the nucleic acids are selected from the group consisting of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), peptide nucleic acid (PNA), locked nucleic acid (LNA), and analogs thereof.

14. The method of claim 11, wherein the template comprises 2 to 10 single-stranded nucleic acids.

15. The method of claim 11, wherein each of the at least two single-stranded nucleic acids comprises 2 to 5 segments.

16. The method of claim 11, wherein the adjacent segments further comprise a linker consisting of 1 to 50 nucleotides that do not complementarily bind to any one of the at least two single-stranded nucleic acids.

17. The method of claim 11, wherein a metal of the metal ion is selected from the group consisting of gold, silver, copper, platinum, palladium, and combinations thereof.