METHODS FOR INDUCING EPITHELIAL CANCER CELL SENESCENCE
First Claim
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1. A method for determining the prognosis of an epithelial ovarian cancer patient, comprising:
- (a) determining the level of expression of a nucleic acid encoding Wnt5a in a tissue sample obtained from the patient, or (b) determining the concentration of Wnt5a protein in a tissue sample obtained from the patient;
(c) comparing the level of expression determined according to step (a) with one or more reference values comprising a Wnt5a nucleic acid expression level that is lower than the Wnt5a nucleic acid expression level in a subject that does not have epithelial ovarian cancer and that is indicative of a poor prognosis for epithelial ovarian cancer, using a processor programmed to compare the level of expression determined according to step (a) with the reference values, or(d) comparing concentration of Wnt5a protein determined according to step (b) with one or more reference values comprising a Wnt5a protein expression level that is lower than the Wnt5a protein expression level in a subject that does not have epithelial ovarian cancer and that is indicative of a poor prognosis for epithelial ovarian cancer; and
,(e) determining the prognosis of the patient based on the comparison.
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Abstract
Systems, methods, and computer readable media for diagnosing or characterizing epithelial cancer or its stages based on the level of expression of the Wnt5a gene or protein are provided. The level of nucleic acids encoding Wnt5a or the level of Wnt5a protein is measured in a tissue sample, and the level is compared with reference values. Methods for inducing senescence of an epithelial cancer cell are also provided.
18 Citations
20 Claims
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1. A method for determining the prognosis of an epithelial ovarian cancer patient, comprising:
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(a) determining the level of expression of a nucleic acid encoding Wnt5a in a tissue sample obtained from the patient, or (b) determining the concentration of Wnt5a protein in a tissue sample obtained from the patient; (c) comparing the level of expression determined according to step (a) with one or more reference values comprising a Wnt5a nucleic acid expression level that is lower than the Wnt5a nucleic acid expression level in a subject that does not have epithelial ovarian cancer and that is indicative of a poor prognosis for epithelial ovarian cancer, using a processor programmed to compare the level of expression determined according to step (a) with the reference values, or (d) comparing concentration of Wnt5a protein determined according to step (b) with one or more reference values comprising a Wnt5a protein expression level that is lower than the Wnt5a protein expression level in a subject that does not have epithelial ovarian cancer and that is indicative of a poor prognosis for epithelial ovarian cancer; and
,(e) determining the prognosis of the patient based on the comparison. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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- 16. A method for inducing senescence in an epithelial ovarian cancer cell having a reduced level of expression of Wnt5a protein, comprising enhancing the expression of the Wnt5a gene in the epithelial ovarian cancer cell to a level of expression that causes one or more of activation of the HIRA pathway in the cell, antagonism of the Wnt canonical signal pathway in the cell, or antagonism of beta-catenin signaling in the cell such that senescence is induced in the cell as a result of one or more of the activation of the HIRA pathway in the cell, the antagonism of the Wnt canonical signal pathway in the cell, or the antagonism of beta-catenin signaling in the cell.
- 18. A method for inducing senescence in an epithelial ovarian cancer cell having a reduced level of expression of Wnt5a protein, comprising contacting the epithelial ovarian cancer cell with an amount of Wnt5a protein, or biologically active fragment thereof, sufficient to increase the level of the Wnt5a protein in the cell to cause one or more of activation of the HIRA pathway in the cell, antagonism of the Wnt canonical signal pathway in the cell, or antagonism of beta-catenin signaling in the cell such that senescence is induced in the cell as a result of one or more of the activation of the HIRA pathway in the cell, the antagonism of the Wnt canonical signal pathway in the cell, or the antagonism of beta-catenin signaling in the cell.
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