FEED ADDITIVE COMPOSITION

• US 20130330307A1
• Filed: 01/19/2012
• Published: 12/12/2013
• Est. Priority Date: 02/18/2011
• Status: Abandoned Application

• Current Assignee: DuPont Nutrition Biosciences ApS (International Flavors & Fragrances Incorporated)
• Original Assignee: DuPont Nutrition Biosciences ApS (International Flavors & Fragrances Incorporated)
• Inventors: Millan, Luis Fernando Romero
• Application Number:
  US13/985,847
• Publication Number:
  US 20130330307A1
• Time in Patent Office: Days
• US Class Current: 424/93.41
• CPC Class Codes: A23K 10/18 of live microorganisms
• CPC Class Codes: A23K 20/189 Enzymes
• CPC Class Codes: A23K 40/10 by agglomeration; by granulation, e.g. making powders
• CPC Class Codes: A23K 40/30 by encapsulating; by coating
• CPC Class Codes: A23K 50/30 for swines
• CPC Class Codes: A23K 50/60 for weanlings
• CPC Class Codes: A23K 50/75 for poultry
• CPC Class Codes: A61P 1/00 Drugs for disorders of the alimentary tract or the digestive system
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 33/02 Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)
• CPC Class Codes: C12N 9/50 Proteinases , e.g. Endopeptidases (3.4.21-3.4.25)
• CPC Class Codes: C12R 2001/125 Bacillus subtilis ; Hay bacillus; Grass bacillus
• CPC Class Codes: C12Y 301/03026 4-Phytase (3.1.3.26), i.e. 6-phytase

Abstract

A feed additive composition comprising a direct fed microbial in combination with a protease, and a phytase, and a method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving the subject'"'"'s resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject, which method comprising administering to a subject a direct fed microbial in combination with a protease and a phytase.

Claims

1. A feed additive composition comprising a direct fed microbial in combination with a protease and a phytase.

2. A feed additive composition according to claim 1 wherein the direct fed microbial is an antipathogen direct fed microbial.

3. A feed additive composition according to claim 1 or claim 2 wherein the direct fed microbial is a viable bacterium.

4. A feed additive composition according to any one of claims 1 to 3 wherein the direct fed microbial comprises a bacterium from one or more of the following genera:

Lactobacillus, Lactococcus, Streptococcus, Bacillus, Pediococcus, Enterococcus, Leuconostoc, Carnobacterium, Propionibacterium, Bifidobacterium, Clostridium and Megasphaera and combinations thereof.

5. A feed additive composition according to any one of the preceding claims wherein the direct fed microbial comprises a bacterium from one or more of the following species:

Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Enterococcus faecium, Enterococcus spp, and Pediococcus spp, Lactobacillus spp, Bifidobacterium spp, Lactobacillus acidophilus, Pediococsus acidilactici, Lactococcus lactis, Bifidobacterium bifidum, Propionibacterium thoenii, Lactobacillus farciminus, lactobacillus rhamnosus, Clostridium butyricum, Bifidobacterium animalis ssp. animalis, Lactobacillus reuteri, Bacillus cereus, Lactobacillus salivarius ssp. salivarius, Megasphaera elsdenii, Propionibacteria sp and combinations thereof.

6. A feed additive composition according to any one of the preceding claims wherein the direct fed microbial is one or more of the following strains:

Bacillus subtilis strains 3A-P4 (PTA-6506);

15A-P4 (PTA-6507);

22C-P1 (PTA-6508);

2084 (NRRL B-500130);

LSSA01 (NRRL-B-50104);

BS27 (NRRL B-50105);

BS 18 (NRRL B-50633); and

BS 278 (NRRL B-50634).

7. A feed additive composition according to any one of the preceding claims wherein the direct fed microbial is in the form of an endospore.

8. A feed additive composition according to any one of the preceding claims wherein the protease is a subtilisin, a bacillolysin, an alkaline serine protease or a keratinase or a Nocardiopsis protease.

9. A feed additive composition according to any one of the preceding claims wherein the phytase is a 6-phytase or a 3-phytase.

10. A feed additive composition according to claim 9 wherein the phytase is a 6-phytase.

11. A feed additive composition according to any one of the preceding claims wherein the phytase is an E. coli phytase or a Buttiauxella phytase or Hafnia phytase or Citrobacter phytase or an Aspergillus phytase or a Penicillium phytase or a Trichoderma phytase or an E. coli phytase or a Hansenula phytase.

12. A feed additive composition according to any one of the preceding claims wherein the phytase is present at a dosage of between 200 FTU/g feed additive composition and 10000 FTU/g feed additive composition.

13. A feed additive composition according to any one of the preceding claims wherein the protease is present at a dosage of 1000 PU/g feed additive composition and 60000 PU/g feed additive composition.

14. A feed additive composition according to any one of the preceding claims wherein the DFM is present at a dosage of 3.75×

10⁷CFU/g feed additive composition and 1×

10¹¹CFU/g feed additive composition.

16. A method according to claim 15 comprising administering the feed additive composition according to any one of claims 1-14.

28. Use according to claim 27 wherein the feed additive composition according to any one of claims 1-14 is used.

40. A kit according to claim 39 wherein said kit comprises the feed additive composition according to any one of claims 1-14.

52. A feed comprising a feed additive composition according to any one of claims 1-14.

53. A feed according to claim 52 wherein the phytase is present at a dosage of between 400 FTU/kg feed and 1000 FTU/kg feed.

54. A feed according to any one of claims 52-53 wherein the protease is present at a dosage of between 2000 PU/kg feed to 6000 PU/kg feed.

55. A feed according to any one of claims 52-54 wherein the DFM is present at a dosage of 7.5×

10⁴CFU/kg feed and 1×

10⁷CFU/kg feed.

56. A method of preparing a feedstuff comprising admixing a feed component with a feed additive composition according to any one of claims 1-14.

58. A premix comprising a feed additive composition according to any one of claims 1-14 in combination with at least one mineral and/or at least one vitamin.

59. A feed additive composition according to any one of claims 1-14 for preventing and/or treating coccidiosis and/or necrotic enteritis in a subject.

60. A method of preventing and/or treating necrotic enteritis and/or coccidiosis wherein an effective amount of a feed additive composition according to any one of claims 1-14 is administered to a subject.

15. A method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving the subject'"'"'s resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject or for reducing populations of pathogenic bacteria in the gastrointestinal tract of a subject, or for reducing nutrient excretion in manure, which method comprising administering to a subject a direct fed microbial in combination with a protease and a phytase.

17. A method according to claim 15 or 16 wherein the direct fed microbial is an antipathogen direct fed microbial.

18. A method according to any one of claims 15-17 wherein the direct fed microbial is a viable bacterium.

19. A method according to any one of claims 15 to 18 wherein the direct fed microbial comprises a bacterium from one or more of the following genera:

Lactobacillus, Lactococcus, Streptococcus, Bacillus, Pediococcus, Enterococcus, Leuconostoc, Carnobacterium, Propionibacterium, Bifidobacterium, Clostridium and Megasphaera and combinations thereof.

20. A method according to any one of claims 15-19 wherein the direct fed microbial comprises a bacterium from one or more of the following species:

Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Enterococcus faecium, Enterococcus spp, and Pediococcus spp, Lactobacillus spp, Bifidobacterium spp, Lactobacillus acidophilus, Pediococsus acidilactici, Lactococcus lactis, Bifidobacterium bifidum, Propionibacterium thoenii, Lactobacillus farciminus, lactobacillus rhamnosus, Clostridium butyricum, Bifidobacterium animalis ssp. animalis, Lactobacillus reuteri, Bacillus cereus, Lactobacillus salivarius ssp. salivarius, Megasphaera elsdenii, Propionibacteria sp and combinations thereof.

21. A method according to any one of claims 15-20 wherein the direct fed microbial comprises a bacterium is one or more of the following strains:

Bacillus subtilis strains 3A-P4 (PTA-6506);

15A-P4 (PTA-6507);

22C-P1 (PTA-6508);

2084 (NRRL B-500130);

LSSA01 (NRRL-B-50104);

BS27 (NRRL B-50105);

BS 18 (NRRL B-50633); and

BS 278 (NRRL B-50634).

22. A method according to any one of claims 15-21 wherein the direct fed microbial is in the form of an endospore.

23. A method according any one of claims 15-22 wherein the protease is a subtilisin, a bacillolysin, an alkaline serine protease or a keratinase or a Nocardiopsis protease.

24. A method according any one of claims 15-23 wherein the phytase is a 6-phytase or a 3-phytase.

25. A method according to claim 24 wherein the phytase is a 6-phytase.

26. A method according any one of claims 15-25 wherein the phytase is an E. coli phytase or a Buttiauxella phytase or Hafnia phytase or Citrobacter phytase or an Aspergillus phytase or a Penicillium phytase or a Trichoderma phytase or an E. coli phytase or a Hansenula phytase.

27. Use of a direct fed microbial in combination with a protease and a phytase for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility) or for improving nitrogen retention) or for improving the subject'"'"'s resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject or for reducing populations of pathogenic bacteria in the gastrointestinal tract of a subject or for reducing nutrient excretion in manure.

29. Use according to any one of claims 27-28 wherein the direct fed microbial is an antipathogen direct fed microbial.

30. Use according to any one of claims 27-29 wherein the direct fed microbial is a viable bacterium.

31. Use according to any one of claims 27-30 wherein the direct fed microbial comprises a bacterium from one or more of the following genera:

Lactobacillus, Lactococcus, Streptococcus, Bacillus, Pediococcus, Enterococcus, Leuconostoc, Carnobacterium, Propionibacterium, Bifidobacterium, Clostridium and Megasphaera and combinations thereof.

32. Use according to any one of claims 27-31 wherein the direct fed microbial comprises a bacterium from one or more of the following species:

Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Enterococcus faecium, Enterococcus spp, and Pediococcus spp, Lactobacillus spp, Bifidobacterium spp, Lactobacillus acidophilus, Pediococsus acidilactici, Lactococcus lactis, Bifidobacterium bifidum, Propionibacterium thoenii, Lactobacillus farciminus, lactobacillus rhamnosus, Clostridium butyricum, Bifidobacterium animalis ssp. animalis, Lactobacillus reuteri, Bacillus cereus, Lactobacillus salivarius ssp. salivarius, Megasphaera elsdenii, Propionibacteria sp and combinations thereof.

33. Use according to any one of claims 27-32 wherein the direct fed microbial is one or more of the following strains:

Bacillus subtilis strains 3A-P4 (PTA-6506);

15A-P4 (PTA-6507);

22C-P1 (PTA-6508);

2084 (NRRL B-500130);

LSSA01 (NRRL-B-50104);

BS27 (NRRL B-50105);

BS 18 (NRRL B-50633); and

BS 278 (NRRL B-50634).

34. Use according to any one of claims 27-33 wherein the direct fed microbial is in the form of an endospore.

35. Use according to any one of claims 27-34 wherein the protease is a subtilisin, a bacillolysin, an alkaline serine protease or a keratinase or a Nocardiopsis protease.

36. Use according to any one of claims 27-35 wherein the phytase is a 6-phytase or a 3-phytase.

37. Use according to claim 36 wherein the phytase is a 6-phytase.

38. Use according to any one of claims 27-37 wherein the phytase is an E. coli phytase or a Buttiauxella phytase or Hafnia phytase or Citrobacter phytase or an Aspergillus phytase or a Penicillium phytase or a Trichoderma phytase or an E. coli phytase or a Hansenula phytase.

39. A kit comprising a direct fed microbial, a protease, a phytase and instructions for administration.

41. A kit according to any one of claims 39-40 wherein the direct fed microbial is an antipathogen direct fed microbial.

42. A kit according to any one of claims 39-41 wherein the direct fed microbial is a viable bacterium.

43. A kit according to any one of claims 39-42 wherein the direct fed microbial comprises a bacterium from one or more of the following genera:

Lactobacillus, Lactococcus, Streptococcus, Bacillus, Pediococcus, Enterococcus, Leuconostoc, Carnobacterium, Propionibacterium, Bifidobacterium, Clostridium and Megasphaera and combinations thereof.

44. A kit according to any one of claims 39-43 wherein the direct fed microbial comprises a bacterium from one or more of the following species:

Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Enterococcus faecium, Enterococcus spp, and Pediococcus spp, Lactobacillus spp, Bifidobacterium spp, Lactobacillus acidophilus, Pediococsus acidilactici, Lactococcus lactis, Bifidobacterium bifidum, Propionibacterium thoenii, Lactobacillus farciminus, lactobacillus rhamnosus, Clostridium butyricum, Bifidobacterium animalis ssp. animalis, Lactobacillus reuteri, Bacillus cereus, Lactobacillus salivarius ssp. salivarius, Megasphaera elsdenii, Propionibacteria sp and combinations thereof.

45. A kit according to any one of claims 39-43 wherein the direct fed microbial is one or more of the following strains:

Bacillus subtilis strains 3A-P4 (PTA-6506);

15A-P4 (PTA-6507);

22C-P1 (PTA-6508);

2084 (NRRL B-500130);

LSSA01 (NRRL-B-50104);

BS27 (NRRL B-50105);

BS 18 (NRRL B-50633); and

BS 278 (NRRL B-50634).

46. A kit according to any one of claims 39-45 wherein the direct fed microbial is in the form of an endospore.

47. A kit according to any one of claims 39-46 wherein the protease is a subtilisin, a bacillolysin, an alkaline serine protease or a keratinase or a Nocardiopsis protease.

48. A kit according to any one of claims 39-47 wherein the phytase is a 6-phytase or a 3-phytase.

49. A kit according to claim 48 wherein the phytase is a 6-phytase.

50. A kit according to any one of claims 39-49 wherein the phytase is an E. coli phytase or a Buttiauxella phytase or Hafnia phytase or Citrobacter phytase or an Aspergillus phytase or a Penicillium phytase or a Trichoderma phytase or an E. coli phytase or a Hansenula phytase.

51. A method of preparing a feed additive composition, comprising admixing a direct fed microbial with a protease and a phytase and (optionally) packaging.

57. A premix comprising a feed additive composition comprising a direct fed microbial in combination with a protease and a phytase, and at least one mineral and/or at least one vitamin.

61. A feed additive composition or feed or kit or method or use or premix as defined generally herein with reference to the Figures and the Examples.